Germination of spores of Clostridium perfringens S-45 was optimal at pH 6.0 at a temperature of 30 C in a medium freed of dissolved oxygen. Heat activation was necessary, and maximal germination occurred when spores were heated for 20 min at 75 C. Of the various materials examined, a combination of cystine and sodium chloride was most effective for inducing rapid and complete germination.
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