INTRODUCTION MATERIALS AND METHODS Viruses, Cells, and Medium iii Propagation of Viruses 42 Primary virus culture 42 Cultivation of Sendai virus 42 Propagation of viruses for vaccine preparation 43 Virus Purification 44 Preparation of Vaccines 45 Production of Hyperimmune Sera 46 Hemagglutination Inhibition Test 46 Radioimmunoprecipitation procedure 54

Salmonella hepatitis is a rare complication of Typhoid fever in tropics. Typhoid fever is an infectious disease associated with high morbidity and mortality. The classical pattern of presentation is continuous fever with a “step ladder”rise, headache, abdominal pain, diarrhea, constipation, and relative bradycardia. In a rare instance, Typhoid fever presented with the feature of acute hepatitis like hepatomegaly and deranged liver functions with hepatic encephalopathy. After taking proper informed consent,we have reported a young patient who presented with low-grade fever, diarrhea,and jaundice. Subsequently he was found to have acute salmonella hepatitis secondary to typhoid fever. Recognition of Salmonella hepatitis is of clinical importance as it can mimic acute viral hepatitis. Early institution of specific therapeutic intervention and supportive care improve the prognosis in these patients.