This work describes the first epidemiological survey ofBurkholderia cepacia involved in pulmonary infections among the Portuguese population with cystic fibrosis (CF) who attended the major CF treatment Center in Lisbon at Sta. Maria Hospital from 1995 to the end of 1997. The characterization of the genomic relatedness of the isolates was based on the analysis of their ribopatterns (withEcoRI) followed by construction of a ribotype-based phylogenetic tree. This study was complemented with macrorestriction fragment analysis by pulsed-field gel electrophoresis. After optimization of the solid growth medium, we found that exopolysaccharide (EPS) production by B. cepacia CF isolates is not as rare a phenomenon as was thought before; indeed, 70% of the isolates examined were EPS producers.

This paper set out to explore the precision agriculture (PA)-training needs of students studying in agricultural universities in the Euro-Mediterranean region (Greece, Italy, Portugal and Spain). SPARKLE is a Knowledge Alliance Project, funded by the European Union (EU), and one of its main goals is to narrow the innovation divide between entrepreneurship and the effective application of sustainable PA. During the project, the research conducted in all countries in the Euro-Mediterranean region revealed differences in the PA-training needs of university students. Additionally, this paper set out to explore the socioeconomic characteristics of students that affect their interest and knowledge towards PA. Finally, this paper aimed to understand the scope, present status and strategies for improving PA training in agricultural universities in the Euro-Mediterranean region. The following descriptive statistics and two multivariate analysis techniques were used: Two-Step Cluster Analysis (TSCA) and Categorical Regression (CATREG). Results support the notion that the lack of “PA knowledge/interest” adds to the technological gap amongst university students, slow adoption of PA and lower levels of overall rural economic development. These findings will be used as the fundamental cognition for the development of a joint action plan and several other national plans in the selected regions.

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Changes on the climate of the boundary layer occur when a vegetation cover above a bare soil is introduced, namely on temperatures and humidity profiles, above and under soil surface. Since air and soil temperatures affect crop growth and development and also soil moisture, they have been used as driving variables in numerous crop growth and development models as well as in those referred to soil mineralization, evaporation, transpiration, etc. The aim of this work was to evaluate (a) the soil thermal behavior in two olive orchards (Olea europaea sp. europaea), both grown on soils with little profile development (Regosol and Cambisol) and subject to drip irrigation but with different spacing between trees, and (b) the air thermal profile over olive rows. Experiments were performed from April to June 2012 in Southern Portugal. Soil and air temperatures were measured by thermocouples. The two orchards changed spatial distribution of soil surface temperature, soil temperature profiles and air temperature within the canopy, either on a daily or hourly basis. Olive tree spacing and irrigation affected both the soil thermal behavior and air thermal profiles. Tree spacing affected the horizontal gradients established along the interrows (intensity and rhythm). Irrigation reduced hourly and daily mean soil surface temperatures and daily thermal amplitudes of both profiles. Differences were also found on damping depths of the thermal wave estimated for the driest and the wettest profiles. Along the row, the effect of shading seems to overlap that of irrigation in a hourly basis.

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