Additional Supporting Information may be found in the online version of this article: Figure S1. The tobacco extracts exhibited concentration-dependent cytotoxicity to melanocyte culture in more than 20 ll/ml by XTT assay using Cell Proliferation Kit 2 (Roche, Basel, Switzerland) after 24 h culture. Figure S2. Melanocytes cultured with tobacco smoke extract and UVB irradiation. Figure S3. Real-time PCR assay showed a tobacco smoke extract concentration-dependent change in MITF expression (A). Abstract: Blue light is a UV-free irradiation suitable for treating chronic skin inflammation, for example, atopic dermatitis, psoriasis, and hand-and foot eczema. However, a better understanding of the mode of action is still missing. For this reason, we investigated whether dendritic cells (DC) are directly affected by blue light irradiation in vitro. Here, we report that irradiation neither induced apoptosis nor maturation of monocyte-derived and myeloid DC. However, subsequent DC maturation upon LPS/IFNc stimulation was impaired in a dosedependent manner as assessed by maturation markers and cytokine release. Moreover, the potential of this DC to induce cytokine secretion from allogeneic CD4 T cells was reduced. In conclusion, unlike UV irradiation, blue light irradiation at high and low doses only resulted in impaired DC maturation upon activation and a reduced subsequent stimulatory capacity in allogeneic MLRs with strongest effects at higher doses.

The Her2-neu score 0 might be considered as an innovative prognostic factor for patients with TNBC indicating poor clinical outcome.