The present work analyzed the stainability and in vitro germinability of the gamma-ray-irradiated fresh and cryopreserved pollens of 3 Citrus species: C. limon, C. limetta, and C. sinensis. Fruit set after pollinating 75 flowers of C. grandis with irradiated and nonirradiated (control) fresh and cryopreserved pollen was also assessed. Results showed that in solid and liquid culture media the highest in vitro germination values were detected in fresh (61.45% and 62.53%, respectively) as well as cryopreserved (53.17% and 55.36%, respectively) C. sinensis nonirradiated pollens. However, at a higher irradiation dose of 500 Gy in both culture media, the minimum reduction of germinability of fresh (30.85% and 28.01%) and cryopreserved pollens (33.37% and 31.06%) was found in C. limon. Contrary to in vitro germination, the minimum reduction in stainability of fresh and cryopreserved pollens, as assessed by acetocarmine (9.51% and 11.85%, respectively) and FDA tests (10.78% and 12.73%, respectively), was recorded in C. limetta. Regardless of irradiation dose, the highest fruit setting in C. grandis at 40 days after pollination was detected when it was pollinated with both fresh (38.02%) and cryopreserved (34.92%) C. limetta pollen grains.
Perennial trees especially fruit trees are considered to be the most competent biological system where atmospheric carbon dioxide is transformed into long-lived soil carbon despite their nutritional and export value. Higher carbon stock helps to sustain production and soil ecosystem services. Better crop nutrition promotes carbon stock. Feasibility of integrated nutrient management in carbon sequestration needs to be evaluated under a subtropical humid condition. An experiment was carried out in randomized block design to study the feasibility of integrated nutrient management for improving soil properties, nutrient availability, fruit yield, and carbon stock in a mango (Mangifera indica L.) (cv. Langra) orchard under a subtropical condition. Various combinations of integrated (farmyard manure, vermicompost, straw mulch, biofertilizers) nutrient management practices were evaluated in two consecutive years in a 30year-old mango orchard. The results revealed that the organic mulching with straw and conjoint application of farmyard manure and vermicompost improve nutrient availability, microbial activeness (29-44%), and carbon stock (~40%) in soil at 0-60 cm soil depth which ultimately improves fruit yield (26-34%). Hence, adoption of integrated nutrient management practices through the application of farmyard manure, vermicompost, and organic mulching with straw would uphold the fruit yield and carbon stock in soil and also promote CO 2 sequestration in soil and less greenhouse gas emission, which paved viable economic options to mitigate climate change.
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