Abstract. This research aims to elucidate the crystallinity, structural and mechanical properties of cotton/spandex knitted fabric after conducting heat setting i.e., annealing process with optimized condition for the first time. Experimental results disclosed that, crystallinity index (CI) (%) of heat-treated cotton/spandex were increased with increased temperature and also mechanical properties i.e., tensile strength was improved as the temperature rose from 120 to 140ºC and decreased with augmenting temperature ranges, which is in good agreement with the findings of CI % through X-ray diffraction (XRD) patterns. The attendance of cellulose dehydration was further confirmed by Fourier transform infrared (FT-IR) spectra in order to observe the structural integrity.
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