The purpose of this study was to verify the effect of the microwave disinfection in denture base acrylic resins with Candida sp biofilm formation. Samples (n=90) of each resin were submitted to ethylene oxide sterilization, and then infected by inoculum of yeast (C. albicans, C. tropicalis and C. dubliniensis) with 200 μL of cultures adjusted to 10 8 CFU/mL, placed in specific media and aerobically incubated at 37°C for 24 hours for biofilm formation. For each resin and microorganism, samples were irradiated by microwave energy at 650 W for 3 minutes. Nonirradiated samples and without microorganisms samples were considered as control. The samples were washed with saline solution and plated in Sabouraud dextrose agar (SDA) for analysis of the number of colony forming units (CFU/mL). Data were submitted to KruskalWallis followed by Student-Newman-Keuls test (α=0.05). The results demonstrated a significant decrease in the number of viable colonies for all biofilms formed by Candida species. It was demonstrated an effective disinfection of the samples contaminated by Candida biofilm when microwave irradiated. This study demonstrated that microwave disinfection was able to significantly decrease the amount of microorganisms from denture base acrylic resins contaminated and therefore could be used as a preventive method of candidiasis caused by dentures.

Rhipicephalus (Boophilus) microplus, known as the cattle tick, is a cause of great economic losses for dairy cattle farming because of its high frequency of occurrence and the difficulty in controlling it. The aim of this study was to evaluate the in vitro activity of Chenopodium ambrosioides extract on R. (B.) microplus. For this purpose, 125 females were selected and classified into five groups according to their weight, in order to ensure that the females used presented homogeneous weight. The treatments comprised 40 and 60% extracts of Chenopodium ambrosioides, distilled water, ethanol (70ºGL) and 12.5% amitraz. The extracts of C. ambrosioides (40 and 60%) showed effectiveness of 99.7 and 100% and higher percentages of dead females than the other treatments: 64 and 96%, respectively (p<0.001). In the groups exposed to distilled water and ethanol (70º GL), 92 and 88% of the females maintained oviposition. In the females exposed to 40 and 60% extracts, oviposition of 36 and 4% occurred, respectively. It was concluded that the extract of Chenopodium ambrosioides, at both concentration evaluated, had high efficiency against engorged females of cattle ticks.