The aims of this study were to test the antifungal activity, toxicity and chemical composition of essential oil from C. sativum L. fruits. The essential oil, obtained by hydro-distillation, was analyzed by gas chromatography/mass spectroscopy. Linalool was the main constituent (58.22%). The oil was considered bioactive, showing an LC50 value of 23 µg/mL in the Artemia salina lethality test. The antifungal activity was evaluated against Microsporum canis and Candida spp. by the agar-well diffusion method and the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were established by the broth microdilution method. The essential oil induced growth inhibition zones of 28 ± 5.42 and 9.25 ± 0.5 for M. canis and Candida spp. respectively. The MICs and MFCs for M. canis strains ranged from 78 to 620 and 150 to 1,250 µg/mL, and the MICs and MFCs for Candida spp strains ranged from 310 to 620 and 620 to 1,250 µg/mL, respectively. C. sativum essential oil is active in vitro against M. canis and Candida spp. demonstrating good antifungal activity.
Evaluation of noni (Morinda citrifolia) volatile profile by dynamic headspace and gas chromatography-mass spectrometry Avaliação do perfil de voláteis em noni (Morinda citrifolia) por headspace dinâmico e cromatografia gasosa-espectrometria de massasAdriana IntroductionNoni comes from the Rubiaceae family which contains approximately eighty species, twenty of which have economic or other benefits. Noni stands out among its other family members because of its diverse uses, and it is able to grow in distinctly different environments including sandy areas, fertile soils, and rocky terrains. Noni is found in most South Pacific islands, Malaysia, the West Indies, Indonesia, the Philippines, Taiwan, Vietnam, India, Africa, and Guam (ELKINS, 2002).The noni plant has traditionally been used by Polynesians for medicinal purposes for more than 2000 years (McCLATCHEY, 2002). The presence of biological compounds has been reported, such as glycosides, polysaccharides, iridoids, alkaloids, lignans, trisaccharide fatty acid esters, anthraquinones, scopoletin, morindin, vitamins, and minerals (WANG et al., 2000;LIU et al., 2001;FURUSAWA et al., 2003;SU et al., 2005). Some in vitro and in vivo analysis demonstrate that noni can confer health benefits in the form of scavenging of free radicals, antimutagenicity, anticarcinoma activity, anticlastogenic activity, inhibition of lowdensity-lipoprotein oxidation, anti-inflammatory activity, blood purification, stimulation of the immune system, regulation of cell function, and regulation of cholesterols (SALUDES et al., 2002;FURUSAWA et al., 2003;HORNICK et al., 2003;KAMIYA et al., 2004). It is noteworthy that according to the Informe Técnico n o 25 (technical safety report #25) noni products commercialization as food should be not allowed in Brazil. This recommendation was based on the lack of scientific information regarding its safety as food (AGÊNCIA..., 2008).Volatile compounds are responsible for the characteristic flavor of foods. These compounds are from different chemical classes present in a wide concentration range and are thermal instable. Commonly, the volatile profile is composed by dozens of compounds, but only a few have an effective contribution to the characteristic food aroma and flavor. Despite its richness in functional compounds, the consumption of noni products could be impaired by their sensory properties. The flesh of the fruit is characteristically bitter, and when completely ripe it produces a very distinctive, somewhat offensive, odor and flavor (ELKINS, 2002). Only one study was found in literature on noni fruit volatile compounds. Farine et al. (1996) analyzed a solvent extract and reported 51 volatile compounds on noni collected in Moorea (French Polynesia). These authors found 25 acids, which corresponded to 83% of the total volatile compounds, mostly low molecular weight fatty acids which are widely known as possessing strong sweat, cheese, fat, and rancid odors. ResumoO noni é um fruto que tem interessado à comunidade científica por sua atividade fun...
*Projeto financiado pela Embrapa-parte da dissertação da primeira autora. PALAVRAS CHAVE ADICIONAISCo-produto. Fibra. Lactação. Pequenos ruminantes. Ricinus communis. ADDITIONAL KEYWORDSByproduct. Fiber. Small ruminant. Ricinus communis. RESUMOObjetivou-se com o presente trabalho, avaliar o efeito dos níveis de substituição do feno de capim Tifton 85 pela casca de mamona na dieta de cabras sobre a produção, composição química e perfil de ácidos graxos do leite. Foram utilizadas 8 fêmeas caprinas Anglo-Nubianas, com peso médio de 45 kg. Os animais foram alojados em baias e distribuídos em delineamento em quadrado latino 4 x 4 duplo. Os tratamentos consistiram de três níveis de substituição do feno de capim Tifton 85 pela casca de mamona como alimento volumoso (0, 33, 67 e 100%) mantendo a relação volumoso:concentrado de 50:50. Cada período experimental teve duração de 21 dias, sendo 14 dias de adaptação e sete dias de coleta de dados. Houve um decréscimo na produção quando a casca de mamona foi inclusa na dieta, sendo que os últimos dois níveis não diferiram entre si. Em relação à composição química do leite, não houve efeito da substituição do feno de capim Tifton 85 pela casca de mamona sobre os teores de proteí-na bruta, matéria mineral, lactose e extrato seco total. Houve aumento do teor de gordura do leite nos níveis 67 e 100% de substituição, porém isso não causou variação na densidade do leite, cuja média foi de 1031. Quanto ao efeito da substituição do feno de capim Tifton 85 pela casca de mamona sobre a concentração dos ácidos graxos, houve efeito sobre os teores dos ácidos mirístico (C 14:0 ), linoelaidico (C 18:2n6t ), linoléico conjugado (CLA 9t11 ) e para o perfil de ácidos graxos saturados (AGSAT), poliinsaturados (AGPOL) e essenciais (AGESS) (p<0,05). Não houve efeito dos tratamentos sobre ácidos graxos insaturados, de cadeia curta, de cadeia longa e monoinsaturados (p>0,05). A substituição do feno de capim Tifton 85 pela casca de mamona reduziu o índice de aterogenicidade (IA) da dieta. De acordo com os resultados obtidos pode-se considerar que a substituição do feno de capim Tifton 85 pela casca de mamona em até 33%, é viável quando o objetivo for melhorar os aspectos qualitativos do leite de cabra. SUMMARYThe aim of this work was to evaluate the effect of substitution levels of the Tifton 85 hay by castor bean hulls in diets for dairy goats on the yield, chemical composition and fatty acids profile of milk. Eight Anglo-Nubian female goats, breed, with average weight of 45 kg were used. The animals were allocated in stalls and distributed in a double
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