A solid phase extraction method for separation and enrichment of uranium(VI) was developed using Penicillium chrysogenum immobilized on silica mixed with double distilled water. Conditions such as pH, sample flow rate and volume, eluting solution and interfering ions with uranium(VI) optimized and determined spectrophotometrically for quantitative sorbtion. Maximum adsorption found at monolayer was 1.3004 mg/g using Langmuir isotherm. The limits of detection (LOD) and limit of quantification (LOQ) of the proposed method for uranium(VI) was 0.61 lg/L and was 2.02 lg/L respectively. The method developed for the separation and recovery of uranium(VI) was applied sediment samples and tap water samples.
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