For the validation of the nifedipine and lignocaine assay by reverse phase high performance liquid chromatography, in both pure form and tablet dosage form, a straightforward, quick, and exact approach has been established. ACN, Methanol, and perchloric acid were used as the mobile phase in chromatography on a Kromasil 100-5-C18 (4.6 x 150 mm, 5 m) column at a flow rate of 1.0 ml/min. 210 nm was used for detection. Nifedipine and lignocaine had retention times of 3.30 and 5.820.02 min, respectively. In the concentration range of 10–50 mg/ml of nifedipine and 20–100 mg/ml of lignocaine, the approach yields linear responses. The method precision for the assay result was less than 2.0%RSD, and the Nifedipine and lignocaine individual assays should be between 98% and 102.0%, respectively. The technique is helpful for pharmaceutical and bulk formulation quality control.
A simple, rapid, sensitive and accurate UV-spectrophotometric method has been developed for the estimation of zileuton in pharmaceutical formulation. The method was developed by using 0.1 N Sodium hydroxide as a solvent and absorbance was measured at 230 nm. The drug exhibited the linearity in the concentration range of 1-6 μg/ml with correlation coefficient of 0.9993. The % recovery of the drug was found to be 98.62 % - 100.5 %. The method was validated as per ICH guidelines. The proposed methods are economical and sensitive for the estimation of zileuton in bulk and tablet dosage form.
A simple, rapid, accurate and precise RP-HPLC method was developed and validated for the determination of zileuton in table dosage form. Chromatographic analysis of the drug was achieved on Cyberlab HPLC comprising of LC- 100P pump, a variable wavelength programmable LC-UV100 UV detector and SCL system controller. Flowrosil C18 column (250 mm x 4.6 mm, 5 μ) as stationary phase with mobile phase consisting of Methanol: Acetonitrile: 1% GAA in the ratio of 70:10:20 v/v. The method showed a good linear response in the concentration range of 5-30 μg/ml with correlation coefficient of 0.9993. The flow rate was maintained at 1.0 ml/min and detection was carried out at 230 nm. The retention time was 3.12 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, solution stability, selectivity and sensitivity. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the determination of zileuton in tablet formulation.
A simple, rapid, sensitive and accurate UV-spectrophotometric method has been developed for the estimation of Famciclovir in pharmaceutical formulation. The method was developed by using 0.1 N HCl as a solvent and absorbance was measured at 312 nm. The drug exhibited the linearity in the concentration range of 2-12 μg/ml with a correlation coefficient of 0.9978. The % recovery of the drug was found to be 98.62 % - 100.5 %. The method was validated as per ICH guidelines. The proposed method is economical and sensitive for the estimation of Famciclovir in bulk and tablet dosage form.
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