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Aims and objectives:This study was carried out to know about the production of biofilm by the microorganisms in various clinical isolates and to compare the antimicrobial sensitivity pattern of biofilm-and nonbiofilm-producing organisms. Materials and methods:One hundred and fifty samples collected from intensive care units for a period of 1 year were taken for the study. Samples included blood, urine, sputum, endotracheal tips, suction tips, pus/swabs, stents/valves, body fluids, etc. Samples were processed and identification of microorganisms and antibiotic sensitivity was tested by methods according to Clinical and Laboratory Standards Institute guidelines.Biofilm production identification was done by tissue culture plate (TCP) method, tube method (TM), and Congo red agar (CRA) plate method.Results: Out of 150 samples, 108 (72%) samples showed growth of Gram-negative bacilli, 16 (11%) samples showed growth of Gram-positive cocci, and Candida species were seen in remaining 26 (17%) samples. Among the total organisms isolated, 124 organisms (82.66%) showed production of biofilm, while 26 organisms (17.33%) did not produce biofilm. Antibiotic resistance was seen more in biofilm-producing organisms as compared with nonbiofilm-producing organisms. Conclusion:Most of the biofilm-related infections are characterized particularly by high resistance to antibiotics and persistent infections, in turn leading to a very high morbidity and mortality. Therefore, detection of biofilm production is of high relevance to the clinician for appropriate approach to the treatment.

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