Bhavana is an Ayurveda process of triturating a powdered drug with herbal juices or decoction. In classical texts this procedure is narrated to have potential to change or initiate positive therapeutic properties in a formulation. Ayurveda literature also shows that if Bhavana of drug juice is given to the powder of same drug then the final product will have few fold more activities than simple powder form of that drug. Present work was undertaken to study this concept on analytical ground as analysis provides basic understanding of changes in chemical composition which is actually related with therapeutic activities. Karavellaka (Momordica charantia Linn.) was chosen as drug for this work as it is well known drug for hyperglycemia. Powder of M. Charantia was made in 6 batches each of 1 kg. Three batches were kept in powder form (Karavellaka Churna, KC) and remaining three batches were given Bhavana of M. Charantia juice (Bhavita Karavellaka Churna, BKC). Physico-chemical analysis along with nutrition value was done to access the proportion of change. All studied parameters showed higher range of values in BKC than KC. Similar result is observed in nutritional value analysis. All these results conclude that Bhavana has synergistic action in increasing concentration of chemical constitutes of the drug. The increase in concentration may reduce the therapeutic dose.
The present work encompasses the application of quality by design principles through the design of experiments for analytical method development using reverse‐phase high‐performance liquid chromatography for estimation of itraconazole as pure drug and from polymeric amorphous dispersion films. A three‐factor three‐level Box Behnken design was used for multivariate analysis of the critical method parameters namely mobile phase composition, flow rate, and pH with a target of achieving a faster retention time of less than 4 min. The optimized method conditions were mobile phase composition of acetonitrile:water in 70:30 ratio, a flow rate of 1 mL/min, and pH of water at pH 7.0. With these method conditions, a retention time of 3.7 min was achieved, and the method was successfully validated to establish linearity in the concentration range of 29.5–129.5 μg/mL with a correlation coefficient of 1.0. The limits of detection and quantification were 0.3 μg/mL and 1.0 μg/mL, respectively. Forced degradation study revealed the ability of the method to detect degradation to oxidation and acidic conditions. This method was used for estimation of drug assay and dissolution from polymeric solid dispersion films prepared at different drug loading concentrations with hydroxypropyl methyl cellulose acetate succinate for accessing formulation variables like polymer type and concentration for improved drug dissolution of Itraconazole.
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