Background: Color vision deficiency or colorblindness, is the inability or reduced ability to distinguish different color spectra, particularly, red & green under normal lighting conditions with unaided eye. Redgreen colorblindness is heritable genetic disorder and most prevalent type of color vision deficiency and its incidence varies between different ethnicity and sex and shows disparity in different parts/regions of the world. Objectives: This study was conducted to find out the prevalence of colorblindness in Eastern part of Ethiopia and identify its distribution among Harari, Oromo and Ethio-Somali ethnic groups. Methods: A total of 2103 (1043 male & 1060 female) students belonging to the three ethnic groups were randomly selected from nine selected primary schools and were screened for color vision deficiency by using Ishihara's tests for color vision deficiency and the data analysis was carried out with SPSS version 16.0. Results: Among those screened for color vision deficiency, 33 of them (1.6%) were diagnosed with colorblindness out of which 31 were male and 2 were female. Out of this; 15, 7 and 11 were from Harari, Oromo and Somali ethnicity respectively. The highest incidence was observed among Harari males (4.2%) and no color vision deficiency was recorded among Oromo females. Deuteronomally was the most frequent color vision defect detected (16, 48.5%) and protanopia was the least detected color vision deficiency with 4 cases (12.1%). Conclusion: Results from this study show similar prevalence rate with previously reported study in Ethiopia for Harari ethnic group but lower prevalence rates for Somali and Oromo ethnic groups. Early detection of colorblindness is important for children to select their future profession and take necessary precautions in their everyday activities. Families and other concerned bodies should also take the case of children with colorblindness into consideration while dealing with them.
Banana is a fruit crop which has high demand in Ethiopia, but its production is constrained by lack of disease free planting material with conventional propagation methods. For shoot initiation, shoot tip explants were cultured on MS medium supplemented with 0.5, 1.0, 1.5 and 2.0 mg/L BAP. Similarly, MS medium supplemented with BAP at 1.0, 1.5, 2.0 mg/L in combination with IBA at 0.25 and 0.50 mg/L were used for shoot multiplication. Half- strength MS medium augmented with IBA at 1.0, 2.0, 3.0 and 4.0 mg/l were used for root induction. MS medium without PGRs were used as controls. Finally, hardening of the in vitro derived plantlets was carried out in green house both in the primary and secondary acclimatization stages. Results showed that the highest shoot initiation percent (93.40%), highest mean number of shoots per explant (4.67) and lesser day for shoot induction (11.00) were observed in explant cultured on MS + 1.0 mg/L BAP. With shoot multiplication, highest shooting percent (92.60%), maximum number of shoots (7.67) and highest shoot length (5.27 cm) were recorded on MS + 1.5 mg/L BAP + 0.5 mg/L IBA. The highest rooting percent (93.40%), maximum root number per shoot (7.67) and highest root length (11.00 cm) were found on a half strength MS medium + 2.0 mg/L IBA. The survival rate of plantlets were 96.00% in coco peat substrate in primary acclimatization and 97.92% in forest soil, sand and manure substrates mixed at 3:2:1 ratio in secondary acclimatization. Overall, the result showed that the PGRs type, concentrations and combinations used are effective for mass propagation of banana variety studied in this experiment.
The environmental stress such as, salinity (soil or water) are serious obstacles for field crops especially in the arid and semi-arid parts of the world. This study was conducted to assess the potential for salt tolerance of cowpea genotypes during the seedling stage. The experimental treatments were 9 cowpea genotypes and 4 NaCl concentrations (0, 50, 100 and 200 mM) and they were tested in greenhouse. The experimental design was completely randomized design in factorial combination with three replications. Data analysis was carried out using SAS (version 9.1) statistical software. Seedling shoots and root traits, seedling shoots and root weight, number of leaves and total biological yield were evaluated. The analyzed data revealed highly significant (p < 0.001) variation among cowpea genotypes, treatments and their interactions. It is found that salt stress significantly decreased root length, shoot length, seedling shoot and root weight of cowpea genotypes. The extent of decrease varied with genotypes and salt concentrations. Most genotypes were highly susceptible to 200 mM NaCl concentration. The correlation analysis revealed positive and significant association among most of the parameters. Genotypes 210856, 211557 and Asebot were better salt tolerant. The study revealed the presence of broad intra specific genetic variation in cowpea varieties for salt stress with respect to their early biomass production.
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