Background:This study targets to optimize and analyse the interactive effects of process variables for improved bioactive metabolite production using RSM and unstructured kinetic modelling by S. halotolerans VSM 2. Materials and Methods: RSM was applied to optimize the interactive effects of five variables, viz., time of incubation, pH, temperature, concentration of maltose and meat extract on bioactive metabolite production and its effect against the five responses viz., S. flexneri, S. marcescens, P. vulgaris, P. aeruginosa and E. coli. Models of Logistic and Luedeking-Piret were used to simulate the cellular increase and bioactive metabolite production. Results: RSM optimal conditions for the bioactive metabolite production recorded were incubation time (12days), pH (8), and temperature (25 0 C), concentrations of maltose and meat extract (1 % w/v) (each). The effect of the bioactive metabolite produced (zone of inhibition) against the responses were found to be 17 mm for S. flexneri, 17 mm for S. marcescens, 16 mm for P. vulgaris, 17 mm for P. aeruginosa and 18 mm for E coli. The data obtained from experimental values are in close agreement with the predicted values of RSM. Model adequacy was evaluated using ANOVA variance where the quadratic effect of p<0.0001 which imply the significance of the model. The unstructured-, mathematical-kinetic models provided a better approximation of profiles of S. halotolerans VSM 2 growth, optimized media utilization and bioactive metabolite production. Conclusion: Optimization of the independent variables for the production of the bioactive metabolite using RSM by S. halotolerans VSM 2 and its effect against the five responses were documented. The predicted values are in good agreement with the experimental values. Unstructured models provided a better approximation of kinetic profiles for bioactive metabolite production by S. halotolerans VSM 2.
The purpose of the present study was concerned with the isolation and characterization of a rare actinobacterial strain designated as VL-RK_09 from a Mango orchard and evaluation of its antimicrobial compounds by GC-MS analysis. Soil dilution plate technique was employed for the isolation of the strain on yeast extract malt extract dextrose (YMD) agar medium. The strain was identified as Arthrobacter kerguelensis based on polyphasic taxonomy. Suitable culture media for the production of antimicrobial metabolites was assessed by inoculating the strain in different ISP (International Streptomyces project) media and non ISP media. The culture broth of the strain grown in best suitable medium (ISP-2) was extracted with different solvents viz., chloroform, ethyl acetate, methanol and acetone. The culture broth inoculated in ISP-2 and extracted with ethyl acetate exhibited strong antimicrobial activity against oppurtunistic pathogenic microorganisms tested. The crude ethyl acetate extract exhibiting high antimicrobial activity was analysed by Gas Chromatography-Mass Spectroscopy to reveal the metabolites produced by the strain and evidenced the presence of 39 compounds according to the available library data, NIST MS Search (ver. 2.0). The results of the present study revealed the production of diversified metabolites by the strain and hence this strain could be a possible source for novel antimicrobial compounds.
Objectives: Optimization, isolation, and characterization of bioactive compounds from Streptomyces lavendulocolor VHB-9 isolated from granite mines of Mudigonda village of Khammam district of Telangana state. Methods:The potent strain was identified as S. lavendulocolor VHB-9 by polyphasic taxonomy. The influence of culture conditions on growth and bioactive compounds production was investigated. Purification of bioactive compounds was done using column chromatography. The structures of the compounds were elucidated on the basis of spectroscopic analysis including Fourier transform infrared, electron spray ionization mass spectrophotometry, 1 H nuclear magnetic resonance (NMR), and 13 C NMR. The antimicrobial activity of the compounds produced by the strain was tested against both Gram-positive and Gram-negative bacteria and fungi in terms of minimum inhibitory concentration.Results: Isolation and identification of two compounds, namely (2R, 3R)-2, 3-Butanediol (B1A), and nonadecanoic acid (B1B). Fraction B4 was isolated partially purified fraction and identified by the gas chromatography-mass spectrometry analysis. B1B compound exhibited the highest activity against Bacillus megaterium, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans when compared to B1A and B4 compounds.
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