Adult stem cells divide to renew the stem cell pool and replenish specialized cells that are lost due to death or usage. However, little is known about the mechanisms regulating how stem cells adjust to a demand for specialized cells. A failure of the stem cells to respond to this demand can have serious consequences, such as tissue loss, or prolonged recovery post injury. Here, we challenge the male germline stem cells (GScs) of Drosophila melanogaster for the production of specialized cells, sperm cells, using mating experiments. We show that repeated mating reduced the sperm pool and increased the percentage of GScs in M-and S-phase of the cell cycle. the increase in dividing GScs depended on the activity of the highly conserved G-proteins. Germline expression of RNA-Interference (RNAi) constructs against G-proteins, or a dominant negative G-protein eliminated the increase in GSC division frequency in mated males. consistent with a role for the G-proteins in regulating GSc division frequency, RNA-i against seven out of 35 G-protein coupled receptors (GPCRs) within the germline cells also eliminated the capability of males to increase the numbers of dividing GSCs in response to mating. Metazoan tissues undergo homeostasis wherein stem cells divide and their daughter cells proliferate and differentiate to replace lost cells. The human hematopoietic stem cells, for example, renew a remarkable number of about one trillion blood cells per day 1,2. Stem cells have to maintain a baseline mitotic activity for the production of daughter cells that account for the daily turnover of differentiated cells. However, whether stem cells can modulate their mitotic activity in response to demands that challenge the system is not fully explored. In some instances, stem cells respond to physiological cues; for example, murine hematopoietic stem cells divide more frequently during pregnancy due to increased oestrogen levels 3. In Drosophila melanogaster, intestinal stem cells initiate extra cell divisions upon ablation of differentiated gut cells, and GSCs modulate their mitotic activity in response to environmental conditions, such as nutrient availability and temperature 4-7. Drosophila is an excellent model for identifying the molecules and mechanisms that regulate and fine-tune tissue homeostasis. A plethora of genetic tools are available for manipulating and monitoring dividing adult stem cells. The small size of the fly, the short generation cycle, and the fairly low costs covering their maintenance allow for high throughput screens. Here, we subjected several thousand male and several million virgin female flies to mating experiments, a task challenging to perform with vertebrate model organisms. We discovered that repeated mating caused a reproducible and significant increase in GSC division frequency in Drosophila wild-type (wt) males. Our analysis revealed that this response to mating was dependent on the activity of G-proteins. Impairing G-protein activity from the germline cells eliminated the ability of the GSCs to in...
The replenishment of specialized cells depends on the activity of stem cells.Recent advances in stem cell research have shown that the germline stem cells (GSCs) in Drosophila melanogaster can increase their mitotic activity in response to mating. Here, we show that this ability to respond to mating is eliminated if the males are mutant for the ABC transporter, White, the genetic background for a plethora of fly lines. Furthermore, we were not able to reproduce previous findings that female flies increase their GSC numbers and mitotic activity upon mating. Our findings underline the importance of careful experimental design and control specimen. that, in turn, undergo a cascade of regulated proliferation and differentiation steps. Stem cells have to adjust their activity to situations of demand. Recent advances in the literature suggests that GSCs in the gonad of Drosophila melanogaster can adjust their mitotic divisions to a variety of factors, including diet, temperature, and mating status (Drummond-Barbosa and Spradling, 2001
Adult stem cells divide to renew the stem cell pool and replenish specialized cells that are lost due to death or usage. However, little is known about the mechanisms regulating how stem cells adjust to a demand for specialized cells. A failure of the stem cells to respond to this demand can have serious consequences, such as tissue loss, or prolonged recovery post injury.Here, we challenge the male germline stem cells (GSCs) of Drosophila melanogaster for the production of specialized cells using mating experiments. We show that repeated mating reduced the sperm pool and accelerated germline stem cell (GSC) divisions. The increase in GSC divisions depended on the activity of the highly conserved G-proteins. Germline expression of RNA-Interference (RNA-i) constructs against G-proteins or a dominant negative G-protein eliminated the increase in GSC divisions in mated males. Consistent with a role for the G-proteins in the regulation of GSC divisions, RNA-i against seven out of 35 G-protein coupled receptors (GPCRs) within the germline cells also eliminated the capability of males to accelerate their GSC divisions in response to mating. Our data show that GSCs are receptive to GPCR stimulus, potentially through a network of interactions among multiple signaling pathways.
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