In this study the humoral and cell mediated immune responses and protective effect induced in BALB/C mice immunized with Brucella melitensis periplasmic proteins precipitated with ammonium sulphate at 50% saturation (BCSP50) or precipitated with ammonium sulphate 70% saturation (BCSP70) were evaluated. Serological results of an enzyme linked immunosorbent assay (ELISA) indicated that the immunoglobulin G increased as a response to inoculation of these antigens. Lymphocyte proliferation (LP) and delayed type hypersensitivity (DTH) also increased due to immunization with BCSP70 and BCSP50. However, immunization of mice with BCSP70 and BCSP50 suppressed splenic infection resulting in reduction in the numbers of CFU per spleen compared to non immunized controls. Overall results suggested that BCSP50 was more effective in inducing protective immunity to Brucella melitensis biovar3 infection than BCSP70. Histopathological examination of mice of group (1) revealed deplesion of white pulp and proliferation of reticuloendothelial cells in spleen, activation of kupffer cells with degenerative changes in hepatocytes and granulomatous reaction in liver, interstitial pneumonia and hyperplastic proliferation of bronchial epithelium in lung, degenerative changes of renal tubules and hypercellularity of glomeruli. While group II and III which were immunized either by BCSP70 or BCSP50 revealed lesions in all specimens were of much moderate nature indicating the protective effect of them against Brucella melitensis with attention that the later has more protective effect.
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