Ultrasonic treatment is adopted as one of the important strategies to accelerate the hydrolysis of waste‐activated sludge (WAS). This study intends to optimize amplitude and time for ultrasonic treatment of WAS using statistically designed experiments that would render a better degree of disintegration, measured in terms of chemical oxygen demand (COD) solubilization. The main and interaction effects of nonlinear regression analysis revealed a significant interaction between amplitude and time over the degree of sludge disintegration. The ultrasonic density of 0.45 W/mL for 30 min favored a 153.84% increase in COD solubilization with a 27.65% degree of sludge disintegration. This condition also favored the minimum specific energy input of 801.58 kJ/g total suspended solids compared to other sonication conditions. Besides, the sludge volume index was reduced by 45% and 26% at the ultrasonic density of 0.45 W/mL for 60 and 30 min, respectively. Microscopic examination of WAS revealed disintegration of bacterial flocs during the ultrasonication process releasing filamentous bacteria with smaller floc sizes. The results from this study indicate that higher ultrasonic density for short time could improve the sludge disintegration with minimum specific energy input.
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