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The microbial communities are an indispensable part of the human defense system and coexist with humans as symbionts, contributing to the metabolic functions and immune defense against pathogens. An ecologically stable vaginal microbiota is dominated by Lactobacillus species, which plays an important role in the prevention of genital infections by controlling the vaginal pH, reducing glycogen to lactic acid, and stimulating bacteriocins and hydrogen peroxide. In contrast, an abnormal vaginal microbial composition is associated with an increased risk of bacterial vaginosis, trichomoniasis, sexually transmitted diseases, preterm labor and other birth defects. This microbial diversity is affected by race, ethnicity, pregnancy, hormonal changes, sexual activities, hygiene practices and other conditions. In the present review, we discuss the changes in the microbial community of the vaginal region at different stages of a female's life cycle and its influence on her reproductive health and pathological conditions.

There is a unique microbial community in the female lower genital tract known as the vaginal microbiota, which varies in composition and density and provides significant benefits during pregnancy, reproductive cyclicity, healthy newborn delivery, protection from preterm birth, infections such as UTIs, bacterial vaginosis, and so on, and improves the efficacy of treatments for vaginal cancers. Methods: It is necessary to know how the vaginal microbiome is composed in order to make an accurate diagnosis of the diseases listed above. A microbiome’s members are difficult to classify, and the way microbial communities function and influence host–pathogen interactions are difficult to understand. More and more metagenomic studies are able to unravel such complexities due to advances in high-throughput sequencing and bioinformatics. When it comes to vaginal microbiota research, we’ll be looking at the use of modern techniques and strategies that can be used to investigate variations in vaginal microbiota in order to detect diseases earlier, better treat vaginal disorders, and boost women’s health. Discussion: The discussed techniques and strategies may improve the treatment of vaginal disorders and may be beneficial for women’s overall health.

The present study focus on biosynthesis of stable silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnO NPs) from the leaf and stem extract of a therapeutic plant Carissa opaca. The visual observation, Fourier Transformed Infrared Spectroscopy (FTIR), Inductively Coupled Plasma analysis (ICP), High Resolution Transmission Electron Microscopy (HRTEM), and Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS) were used to characterize and confirm the synthesized AgNPs and ZnO NPs. Afterwards; the synthesized nanoparticles were used to analyze their antimicrobial activity via in-vitro disk diffusion method against Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Aspergillus niger, and Candida albican. Both the nanoparticles showed maximum zone of inhibition against Pseudomonas aeruginosa (bacterial strain), whereas in the case of fungi, higher zone of inhibition was observed using ZnONPs against Candida albican and AgNPs against Aspergillus niger. The biosynthesized AgNPs was also used for degradation of methylene blue under visible-light irradiation and found dye removal efficiency of. 97.4% within 1 h.