G. 1991. Distinct sequence elements in a napin promoter interact in vitro with DNA-binding proteins from Brassica napus. -Physiol. Ptant. 82: 205-212.Nuclear extracts obtained from developing seeds of oilseed rape, Brassica napus cv. Svalov Karat K2O516, were shown to contain severai distinct DNA-binding proteins as evidenced by gel retardation experiments. Four of the proteins were capable of interacting in vitro with oligonucleotide probes containing sequences reiated to motives in a napin gene promoter atsd its upstream region. Another protein interacted with an A/T-rich repeated sequence present at the 3' end of the gene. The proteins appear to be present also in leaf nuclei and do not show any deteaable variations that correlate with napin expression during seed development. Thus, analogous with many transcription factors, the DNA-binding proteins that we have identified are present in both expressing and non-expressing cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.