Juniperus sabina is an interesting species for forest restoration and ornamental purposes. The seeds of this plant have several types of dormancies; therefore, seed propagation is difficult and time consuming. The production of cuttings can be an alternative way to produce plants more quickly. The main objective of this experiment was to determine the best propagation conditions (indole butyric acid dose, substrate, and season) for this species using stem cuttings. Rooting performance of the cuttings was evaluated based on the rooting percentage (%), root biomass, and specific root length (SRL). In addition, we examined the internal composition (auxin and peroxidase content) in treated stem cuttings. Cuttings were pretreated with five doses of indole butyric acid (IBA; 0 (control), 1000, 2000, 4000, and 8000 ppm) and were rooted in four substrates (perlite, perlite-cocopeat, pumice, and mixed substrate) during the four seasons (winter, spring, summer, and autumn). The best treatments, with more than 60% rooting, were applied in spring, and IBA at 1000 ppm in perlite–cocopeat substrate obtained 62% rooting. The highest rooting percentage correlated with the highest root biomass production and the lowest SRL. IBA pretreatment decreased the concentration of peroxidase in spring (coinciding with maximum rooting), representing an indicator of rooting performance. Based on these results, we recommend a new protocol for Juniperus sabina production: (i) prepare cuttings in spring, (ii) treat cutting bases with 1000 ppm IBA, and (iii) plant cuttings in a substrate of perlite–cocopeat (1:1).

The Endemic Juniper of Maymars (Juniperus sabina) is one of the most valuable plants in forested areas. The objectives of this experiment were: I) to determine the best conditions for stem cutting propagation of this species, and II) to examine changes in some of the secondary metabolites during the four months (the first of each season): January, April, July, and October, after rooting of cuttings. The research was done with the treatment of five levels of Indole Butyric Acid, including: 0, 1000, 2000, 4000, and 8000 ppm in four rooting substrates, including perlite, perlite-cocopeat (1:1), pumice, and a mixed rooting substrate (sand, perlite, cocopeat, vermicompost, and potash; 1:1:1:1:1) in the four seasons of the year, with stem cuttings having an average length of 15 cm. The best treatment with more than 50% rooting was seen in April at levels of 4000 and 1000 ppm, and the best substrate was perlite cocopeat. Using lower levels of IBA led to a reduction in total phenol content in the cuttings during the rooting period. The flavonoid content of the cuttings varied across different seasons. Based on these results, we recommend this way of propagation for Juniperus sabina production. This propagation method takes less time in comparison with sexual propagation from seed.