The human CMV (HCMV) genome consists of an approximately 230-kb dsDNA and is predicted to contain over 165 open reading frames. Although the entire sequence of the laboratory-adapted AD169 strain of HCMV was first available in 1991, the precise number and nature of viral genes and gene products are still unclear. Fewer than 100 predicted genes have been convincingly elucidated with respect to their expression patterns, transcript structure and transcription characteristics. The high gene number of HCMV creates a crowded genome with many overlapping transcriptional units. 3´- or 5´-coterminal overlapping polycistronic transcripts could use a common promoter element or a poly-A signal. 3´-coterminal monocistronic transcripts could encode 'nested' open reading frames, which possess different initiation but the same termination sites. As a virus with eukaryotic cells as the host, HCMV has the capacity to splice out introns during transcription. Major alternately spliced mRNA species of HCMV originate primarily, but not exclusively, from the immediate early gene regions. Alternate splicing patterns of the mRNAs could encode a number of gene products with different sizes. In recent years, some antisense and noncoding transcripts of HCMV have been reported. These RNAs probably have functions in genomic replication or the regulation of gene expression.

Abstract:The in situ application of recycled aggregate concrete (RAC) is of great significance in environmental protection and construction resources sustainability. However, it has been limited to nonstructural purposes due to its poor mechanical performance. External confinement using steel tubes and fiber-reinforced polymer (FRP) can significantly improve the mechanical performance of RAC and thus the first-ever study on the axial compressive behavior of glass FRP (GFRP)-confined RAC was recently reported. To have a full understanding of FRP-confined RAC, this paper has extended the type of FRP and presents a systematic experimental study on the axial compressive performance of carbon FRP (CFRP)-confined RAC. The mechanical properties of CFRP-confined RAC from the perspective of the failure mode, ultimate strength and strain, and stress-strain relationship responses were analyzed. Integrated with existing experimental data of FRP-confined RAC, the paper compiles a database for the mechanical properties of FRP-confined RAC. Based on the database, the effects of FRP type (i.e., GFRP and CFRP) and the replacement ratio of recycled coarse aggregate were investigated. The results indicated that the stress-stain behavior of FRP-confined RAC depended heavily on the unconfined concrete strength and the FRP confining pressure instead of the replacement ratio. Therefore, this study adopted eleven high-performance ultimate strength and strain models developed for FRP-confined normal aggregate concrete (NAC) to predict the mechanical properties of FRP-confined RAC. All the predictions had good agreement with the test results, which further confirmed similar roles played by FRP confinement in improving the mechanical properties of RAC and improving those of NAC. On this basis, this paper finally recommended a stress-strain relationship model for FRP-confined RAC.

BackgroundThe genome of human cytomegalovirus (HCMV) has been studied extensively, particularly in the UL/b' region. In this study, transcripts of one of the UL/b' genes, UL144, were identified in 3 HCMV isolates obtained from urine samples of congenitally infected infants.MethodsNorthern blot hybridization, cDNA library screening, and RACE-PCR were used.ResultsWe identified at least 4 differentially regulated 3'-coterminal transcripts of UL144 in infected cells of 1,300, 1,600, 1,700, and 3,500 nucleotides (nt). The 1600 nt transcript was the major form of UL144 mRNA. The largest transcript initiated from the region within the UL141 open reading frame (ORF) and included UL141, UL142, UL143, UL144, and UL145 ORFs.ConclusionsThese findings reveal the complex nature of the transcription of the UL144 gene in clinical isolates.

BackgroundIt has been predicted that the UL31 gene originates from the positive strand of the human cytomegalovirus (HCMV) genome, whereas the UL30 and UL32 genes originate from the complementary strand. Except for the UL32 gene, the transcription of this gene region has not been investigated extensively.MethodsNorthern blotting, cDNA library screening, RACE-PCR,and RT-PCR were used.ResultsAt least eight transcripts of the antisense orientation of UL31 were transcribed from the UL30–UL32 region during the late phase of HCMV infection. The 3′ coterminus of these transcripts was located within the predicted UL30 gene. The longest 6.0-kb transcript was initiated upstream of the predicted UL32 gene. Other transcripts were derived from the predicted UL30 and UL31 gene region. Except for the previously predicted UL32 open reading frame (ORF), three novel ORFs, named UL31anti-1, UL31anti-2 and UL31anti-3, were located in the transcripts from the UL31anti-UL32 transcription unit. No transcription was found in UL31.ConclusionA family of novel 3′ coterminal transcripts was transcribed from the UL30–UL32 gene region.

The functions of some proteins encoded by human cytomegalovirus (HCMV) UL/b genes have been studied; however, systematic analysis of the transcripts for this region is still insufficient. The results of both rapid amplification of cDNA ends (RACE) and cDNA library screening in this study proved that 3 termini of all transcripts in the UL138-UL145 region were located approximately 20 bp downstream from each potential poly (A) signal, which were at the positions of nucleotides 7184, 9954 and 12848 in the UL/b sequence of the H strain, respectively. Thus, there were at least two large families of polycistronic transcripts in this gene region. The first family of 3 -coterminal transcripts contained UL139, UL140 and UL141 genes, and the second one consisted of UL142, UL143, UL144 and UL145 genes. The 3 -coterminal characterization further confirmed that multiple uses of polyadenylation signals were commonly used by HCMV to utilize genetic information.