The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the formation of empty spheres covered by epithelial cells is observed in first 24 h of culture. The growth and survival of organoids are supported by the addition of R-spondin 1, Noggin, and prostaglandin E to the culture medium. The organoids are accompanied by myofibroblasts which become visible in the next 2 days of culture. The intestinal enteroids (free of myofibroblasts) can be obtained from adult chicken intestine.
In Figure 4 Section A, the upper right corner should read "3d", whereas it was incorrectly printed as "4d." Also, the caption of Figure 5 should read: "The cultures were photographed on the first, second and fifth day of culture (1d, 2d, 5d) with differential interference contrast optics." rather than the incorrect "The cultures were photographed on second, third, and fifth day of culture (2d, 3d, 5d) with differential interference contrast optics". These issues have now been corrected.
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