Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition
and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus
(silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant
bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in
cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the
in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to
suppress PGF2α production in a dose-dependent manner. When in
vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa
medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10
ng/ml) significantly promoted development to the
expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same
bioactivity as native IFNτ.
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