Research was conducted at Al-Qasim Green University in the Plant Tissue Culture Laboratory in the Department of Horticulture and Landscaping in the College of Agriculture from November 2021 to May 2022 with the aim of comparing two types of cytokinins: BAP at a concentration of 2.0 mg/L-1 and TDZ at a concentration of 0.2 mg/L-1 and two concentrations Iron (2.5-5 mg/L-1). To know their effect on the average of shoot multiplication in vitro of the root and its content of protein and macro (N.K) and Micro (iron) elements. The research was implemented as a factorial experiment using a complete random design (CRD) with two factors: cytokinin and nano iron. The results indicated a superiority of 0.2 mg.L-1 TDZ in each of the Multiplication shoots (4.667 shoots/explant ) and the content of shoots of iron (54.66 mg.kg-1), while BAP at a concentration of 2.0 mg/L-1 was excelled on the content of shoots of nitrogen, potassium and protein (1.778%, 1.947%.) respectively. As for nano iron at a concentration of 2.5 mg. L-1 was significantly excelled in the average of multiplication shoots (5 shoots/explant ), nitrogen (1.765%) and protein (10.98%). Specifically, the 5 mg dose. Potassium and iron (1.692% and 55.0 mg.kg-1) were most abundant in L-1.
The experiment was conducted in the plant tissue culture laboratory of the Department of Horticulture and Landscape Engineering, College of Agriculture, Al-Qasim Green University. For the purpose of evaluating the tolerance of celery cultivar Festival to water stress in vitro using three concentrations of polyethylene glycol (PEG) (4, 0.2 mg L-1) and three concentrations of nano selenium (Se) (4, 2, 0 mg L-1) in the growth and multiplication of strawberry shoots. The apical meristem taken from the purlins of the aforementioned cultivar, which was obtained from Najaf Research Department - Agricultural Research Department - Iraqi Ministry of Agriculture, was cultivated in the MS medium (Murashige and Skoog) supplied with (1 mg L-1BA + 0. mg L-1). IBA + 80 mg L-1adenine sulfate) for 4 weeks, Then the percentage of surviving plants, the average number of shoots formed, their lengths, the number of leaves, and their fresh and dry weights were calculated as indicators of their response and tolerance to water stress. The results showed a significant reduction in the percentage of plant survival, the number of branches and their lengths, the number of leaves, and their fresh and dry weights when the concentration of polyethylene glycol (PEG) in the multiplication medium was increased. The lowest values were recorded at a concentration of 4 mg L-1compared to the highest results when the control treatment was 0 mg L-1. As for the nano selenium, the highest values were recorded at a concentration of 4 mg L-1compared to the lowest results when the comparison treatment was 0 mg L-1.
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