We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.

Highlights d Binding of ATP-competitive inhibitors to c-Src relieves its auto-inhibition d Inhibitor-bound c-Src in an open state forms a complex with FAK d Reduction of inhibitor concentration activates c-Src and the FAK-Grb2-Erk cascade d Inhibitors activate drug-resistant c-Src mutant and the cascade

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In the version of this article initially published, the sequence reported for the dBrainbow construct was incorrect. The blue fluorescent protein was reported as EBFP2; it is mTFP1. The mKO2 protein is tagged with a V5 epitope and not a Myc epitope, and the EGFP protein is tagged with HSV, not with V5, as detailed in a previous correction to this paper. The red channel reflects endogenous mKO2 fluorescence, not anti-Myc staining. The mKO2 protein is produced by a codon-optimized sequence. These errors have been corrected in the HTML and PDF versions of the article, and the correct sequence is provided.Erratum: Rational design of a high-affinity, fast, red calcium indicator R-CaMP2 In the version of this article initially published, GenBank/EMBL/DDBJ accession codes for R-GECO2L and R-CaMP2 were omitted. These accession codes are KR149061 and KR149062, respectively. The error has been corrected in the HTML and PDF versions of the article.Erratum: Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques In the PDF version of this article initially published, the Acknowledgments section erroneously listed the Uehara Memorial Foundation as a funder twice. The error has been corrected in the PDF version of this article.Erratum: Power and sample size In the version of this article initially published, the terms "sensitivity" and "specificity" and the related descriptors "sensitive" and "specific" were mistakenly switched in three instances. The errors have been corrected in the HTML and PDF versions of the article.

Highlights d Binding of ATP-competitive inhibitors to c-Src relieves its auto-inhibition d Inhibitor-bound c-Src in an open state forms a complex with FAK d Reduction of inhibitor concentration activates c-Src and the FAK-Grb2-Erk cascade d Inhibitors activate drug-resistant c-Src mutant and the cascade