The mechanism by which extracellular molecules control serotonergic cell fate remains elusive. Recently, we showed that noggin, which inactivates bone morphogenetic proteins (BMPs), induces serotonergic differentiation of mouse embryonic (ES) and induced pluripotent stem cells with coordinated Address correspondence and reprint requests to Dr Hitoshi Hashimoto, Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan. E-mail: hasimoto@phs.osaka-u.ac.jp 1 These authors contributed equally to this work. Abbreviations used: 5-HT, 5-hydroxytryptamine/serotonin; AcGFP, Aequorea coerulescens green fluorescent protein; BMP, bone morphogenetic protein; ePet, enhancer of the mouse Pet-1 (Fev) gene; ES, embryonic stem; FBS, fetal bovine serum; FGF, fibroblast growth factor; GLuc, Gaussia luciferase; Id, inhibitor of differentiation; iPS, induced pluripotent stem; MAP2, microtubule-associated protein 2; MEM, minimum essential medium; Sert, serotonin transporter; Shh, sonic hedgehog; TGF-b, transforming growth factor-b; Tph2, tryptophan hydroxylase 2; TuJ1, mouse monoclonal anti-class III-tubulin antibody.