Vitamin E is a natural antioxidant and its most common biologically active form is α-tocopherol. The antiproliferative effects of α-tocopherol have been previously demonstrated. In this study the antimutagenic effects of vitamin E on oncology and non oncology hospital nurses was investigated. A total of 138 female nurses from oncology and non oncology hospitals participated in the study. They received 200 mg/day vitamin E for 2 weeks. The urine samples before and after intake of vitamin E were collected and the nucleus of urothelial cells were evaluated with comet assay. The length of epithelial cells nuclei correlated with increased fracture rate of DNA. Nucleolus length of urine epithelial cells of all nursing staff before and after vitamin E treatment were measured and the data were evaluated by student t-test and SPSS. Our study showed that 20% of nursing staff have apoptosis and DNA fracture in the nucleolus of their urine epithelial cells and DNA damage in the urothelial cells of exposed nurses was significantly higher than the control group (P<0.05).The antimutagenic activity of vitamin E had significant effects on oncology hospital nurses effectively in repairing DNA damage and decreasing their nucleus length in urine epithelial cells.We propose that the higher therapeutic doses of vitamin E and increasing the length of treatment period will be effective against DNA strand breakage and may have more effect on oncology nurses.
Introduction: The aim of this study is to determine the antimutagenic effects of Vitamin E among nurses of oncology and non-oncology hospitals exposed to chemotherapy drugs. Several studies have demonstrated that nurses occupationally exposes to cytostatic drugs. Material and Methods:A total of 138 female nurses from oncology and non-oncology hospitals participated in the study. All urine samples of nurses before and after Vitamin E consumption (200 mg/day) were evaluated by Ames Salmonella typhimorium mutagenicity test using histidine negative of tester strain TA100 with and without S-9mix. In all steps the collected urine samples extracts were prepared using amberlit XAD-2 resins and examined for mutagenicity activity. The data of Ames assay were analyzed with Anova one way and t-test statistical. Results:In the present study 25% of oncology nursing staff excrete carcinogenic compounds in their urine and oral consumption of Vitamin E for two weeks showed significant anti-mutagenic effects.Discussion: It was appeared that the urinary mutagenic activity will decrease by receiving Vitamin E. However, after Vitamin E consumption there was significantly depletion of urinary mutagenic activity in urine extracts among the exposed nursing personnel. Conclusion:We conclude that mild effects of Vitamin E against poor safety and significant adverse events among nurses handling cytotoxic drugs. There is, therefore, a need to improve the safety of the work environment, make available protective equipment, develop standard practice guidelines for oncology nurses and higher therapeutic doses of Vitamin E may be a promising compound for reducing mutagenic effects of antineoplastic drugs among oncology hospital nurses.
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