In this study, we sought to apply the model identity technique to compare the influence of eight treatments on growth parameters for three broiler quail lines, estimated using a logistic nonlinear regression model. For the analysis, we used the weight and age data obtained for three lines of European broiler quails (Coturnix coturnix coturnix) in a completely randomized 2×4 factorial scheme, with two levels of metabolizable energy (2900 and 3100 kcal of ME kg-1 of diet), four levels of raw protein (22%, 24%, 26% and 28% crude protein), and six repetitions. Results obtained for model identity tests indicated that although there were no significant differences among the parameters of the model between the treatments evaluated in each strain, there were, with the exception of Treatment 5 (3100 kcal of ME kg-1 and 22% crude protein), significant differences with respect to the adult weight parameter between lines within each treatment.

The aim of this study was to evaluate the effect of dietary lysine on performance, protein deposition and respiratory chain gene expression in male broilers. A total of 252 Cobb 500 broilers were distributed, in a completely randomized design, into four treatments with seven replicates of nine birds per experimental unit. Experimental treatments consisted of diets based on corn and soybean meal, with four levels of digestible lysine: 1.016%, 1.099%, 1.182% and 1.265%. The increase in the level of digestible lysine in the diet provided higher weight gains, feed efficiency and body protein deposition. Birds fed the lowest level of dietary lysine (1.016%) showed a lower expression of genes such as NADH dehydrogenase subunit I (ND1), cytochrome b (CYTB) and cytochrome c oxidase subunits I (COX I), II (COX II) and III (COX III), displaying the worst performance and body protein deposition. This demonstrates the relationship existing between the expression of the evaluated genes and the performance responses. In conclusion, results indicate that broilers fed diets with higher levels of digestible lysine have increased messenger RNA expression of some genes coded in the mitochondrial electron transport chain (ND1, CYTB, COX I, COX II and COX III). It may be stated that diets with proper levels of digestible lysine, within the 'ideal protein' concept, promote the expression of genes, which increases the mitochondrial energy, thereby fostering body protein deposition and the performance of broilers in the starter phase.

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The objective of this study was to compare non-linear models fitted to the growth curves of quail to determine which model best describes their growth and check the similarity between models by analyzing parameter estimates.Weight and age data of meat-type European quail (Coturnix coturnix coturnix) of three lines were used, from an experiment in a 2 × 4 factorial arrangement in a completely randomized design, consisting of two metabolizable energy levels, four crude protein levels and six replicates. The non-linear Brody, Von Bertalanffy, Richards, Logistic and Gompertz models were used. To choose the best model, the Adjusted Coefficient of Determination, Convergence Rate, Residual Mean Square, Durbin-Watson Test, Akaike Information Criterion and Bayesian Information Criterion were applied as goodness-of-fit indicators. Cluster analysis was performed to check the similarity between models based on the mean parameter estimates. Among the studied models, Richards’ was the most suitable to describe the growth curves. The Logistic and Richards models were considered similar in the analysis with no distinction of lines as well as in the analyses of Lines 1, 2 and 3.

This study aimed to evaluate stability and recommend reference genes for quantitative real-time PCR in different tissues from male and female broiler quails. The stability of 10 housekeeping genes (GAPDH, RPL5, MRPS27, MRPS30, TFRC, HMBS, EEF1, LDHA, B2M and UBC) was analyzed in heart, thigh muscle, brain and spleen, by means Bestkeeper, NormFinder, GeNorm softwares with ∆Cq method. The most stable housekeeping genes were MRPS30, TFRC and HMBS in heart; MRPS30, EEF1 and HMBS in thigh muscle; B2M, GAPDH and UBC in brain; and EEF1, LDHA and HMBS in spleen. It is recommended to be used as reference genes for gene expression studies of male and female quails.

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This study aimed to evaluate stability and recommend reference genes for quantitative real-time PCR in different tissues from male and female broiler quails. The stability of 10 housekeeping genes (GAPDH, RPL5, MRPS27, MRPS30, TFRC, HMBS, EEF1, LDHA, B2M and UBC) was analyzed in heart, thigh muscle, brain and spleen, by means Bestkeeper, NormFinder, GeNorm softwares with ∆Cq method. The most stable housekeeping genes were MRPS30, TFRC and HMBS in heart; MRPS30, EEF1 and HMBS in thigh muscle; B2M, GAPDH and UBC in brain; and EEF1, LDHA and HMBS in spleen. It is recommended to be used as reference genes for gene expression studies of male and female quails.