RESUMO Produção e atividade fotossintética de alface Mimosa Verde e Roxa em dois sistemas de cultivoA alface (Lactuca sativa L.) é a hortaliça folhosa mais consumida na alimentação do brasileiro, sendo boa fonte de vitaminas e de sais minerais. É largamente produzida no sistema convencional. No entanto, o cultivo hidropônico vem ganhando espaço no mercado, conquistando a confiança dos consumidores, cada vez mais exigentes com a qualidade dos alimentos. O objetivo deste trabalho foi avaliar o desempenho de duas cultivares de alface, nos sistemas de cultivo hidropônico e convencional, para a produção de massa fresca (MF) e massa seca (MS), fotossíntese, teores de Lettuce (Lactuca sativa L.) is the most commonly consumed leaf vegetable in the Brazilian diet, and it is a good source of vitamins and minerals. It is widely grown in the conventional farming system. However, the hydroponic farming system has been gaining importance in the market, wining confidence from consumers, who are becoming increasingly more demanding on food quality. The objective of this study was to evaluate the performance of two lettuce cultivars on hydroponic and conventional farming systems for the production of fresh mass (FM) and dry mass (DM), photosynthesis, contents of chlorophyll and anthocyanin. The following two experiments were carried out: hydroponics farming (HF) and conventional farming (CF), performed in protect and unprotect environments, respectively, in Florianópolis, SC. Mimosa Verde cultivar (MV) showed greater fresh mass than Mimosa Roxa (MR), in both farming systems and the two cultivars presented better performance in the hydroponic system (287.7 g MV and 139.1 g MR) than the conventional system (129.7 g MV and 111.8 g MR). Mimosa Verde cultivar presented lower average contents of total chlorophyll (7.7 mg g -¹ FM) than Mimosa Roxa (11.8 mg g -¹FM), and both cultivars displayed higher means for this variable in the hydroponic farming system. Mimosa Roxa presented higher contents of anthocyanin in the conventional system (88.24 mg g -¹ FM) than the ones in the hydroponic system (36.89 mg g -¹ FM). The best results for CO 2 net assimilation rate regarded to photosyntheticaly active photon flux density were found in the hydroponic system, for both lettuce cultivars. Variation in the contents of chlorophyll were also found. Those variations were higher in the protected system than in the hydroponic system and contents of anthocyanin were higher in the conventional system.