Regenerating hair follicles (HFs) is a critical medical need for patients who suffer from serious hair loss. To generate equivalent hair-bearing skin systems that could mimic the complexity of native tissues with pluripotent stem cells, floating culture has been employed as a standard method; however, it is still necessary to improve limitations such as the heterogeneity of organoids and the difficulty of handling them, which increases during long-term culture. Here, we devise a floating-adherent combinatory culture system to establish skin HF organoids from human-induced pluripotent stem cells (hiPSCs). Specifically, embryoid bodies were generated in a free-floating environment, followed by the induction process, which occurred in adherent conditions. With our approach, hair germ-like buds were shown to protrude and extend faster. After 100 days of culture, mature cystic skin organoids stratified to form the epidermis, dermis, and outer root sheath, as evident from quantitative polymerase chain reaction and immunohistochemistry analysis. Dermal condensate cells (Sox2+, PDGFRα+, P75+), which are the precursors of HFs, were detected together with HF stem cells (NFATC1+, LGR5+), putative bulge stem cells (LHX2+, KRT15+) and melanocytes (PMEL+). Notably, our constructed HFs could recapitulate the sensory function of native tissues, as illustrated by the formation of a network of sensory neurons and Schwann cells connecting towards HF cells and epidermal progenitors. In summary, our results demonstrate a new protocol for the simplified and efficient induction of skin HFs from hiPSCs, thereby contributing to research on optimizing HF growth and investigating novel therapeutic strategies to treat alopecia.
The purpose of this study is to evaluate the factors affecting the perception of professional ethics of accounting students at universities in Da Nang City, Vietnam. Through the use of the mixed research method, the authors found that there are 3 factors that affect the perception of professional ethics of the accounting students, respectively: the law of the industry. Education program and Personalization. Through this study results to propose solutions to raise awareness about the professional ethics of accounting students at universities in Da Nang city.
Received: 15 July 2021 / Accepted: 6 October 2021 / Published: 5 November 2021
The purpose of this study is to evaluate the factors affecting the decision to deploy a credit management system at commercial banks in Da Nang city. The author used a combination of mixed research methods in this study. The qualitative research method aims to build a research model from the analysis of previous related studies. The author used a quantitative method to survey 336 subjects who are managers, direct employees of the credit department at banks. Research results show that there are four factors that greatly influence the decision to choose a bank. Deposit individual customer savings in order: usefulness, controllability, relevance, and implementation cost. Through this result, it will help commercial banks orient the development of LOS credit management system at commercial banks in the future.
Received: 10 January 2022 / Accepted: 25 June 2022 / Published: 5 July 2022
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