Ongoing COVID-19 outbreak has raised a drastic challenge to global public health security. Most of the patients with COVID-19 suffer from mild flu-like illnesses such as cold and fever; however, few percentages of the patients progress from severe illness to death, mostly in an immunocompromised individual. The causative agent of COVID-19 is an RNA virus known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Despite these debilitating conditions, no medication to stop the disease progression or vaccination is available till now. Therefore, we aimed to formulate a multi-epitope vaccine against SARS-CoV-2 by utilizing an immunoinformatics approach. For this purpose, we used the SARS-CoV-2 spike glycoprotein to determine the immunodominant T-and B-cell epitopes. After rigorous assessment, we designed a vaccine construct using four potential epitopes from each of the three epitope classes such as cytotoxic T-lymphocytes, helper T-lymphocyte, and linear B-lymphocyte epitopes. The designed vaccine was antigenic, immunogenic, and non-allergenic with suitable physicochemical properties and has higher solubility. More importantly, the predicted vaccine structure was similar to the native protein. Further investigations indicated a strong and stable binding interaction between the vaccine and the toll-like receptor (TLR4). Strong binding stability and structural compactness were also evident in molecular dynamics simulation. Furthermore, the computer-generated immune simulation showed that the vaccine could trigger real-lifelike immune responses upon administration into humans. Finally, codon optimization based on Escherichia coli K12 resulted in optimal GC content and higher CAI value followed by incorporating it into the cloning vector pET28þ(a). Overall, these results suggest that the designed peptide vaccine can serve as an excellent prophylactic candidate against SARS-CoV-2.
Atmospheric particulate matter (PM) has major threats to global health, especially in urban regions around the world. Dhaka, Narayanganj and Gazipur of Bangladesh are positioned as top ranking polluted metropolitan cities in the world. This study assessed the performance of the application of hybrid models, that is, Autoregressive Integrated Moving Average (ARIMA)-Artificial Neural Network (ANN), ARIMA-Support Vector Machine (SVM) and Principle Component Regression (PCR) along with Decision Tree (DT) and CatBoost deep learning model to predict the ambient PM2.5 concentrations. The data from January 2013 to May 2019 with 2342 observations were utilized in this study. Eighty percent of the data was used as training and the rest of the dataset was employed as testing. The performance of the models was evaluated by R2, RMSE and MAE value. Among the models, CatBoost performed best for predicting PM2.5 for all the stations. The RMSE values during the test period were 12.39 µg m−3, 13.06 µg m−3 and 12.97 µg m−3 for Dhaka, Narayanganj and Gazipur, respectively. Nonetheless, the ARIMA-ANN and DT methods also provided acceptable results. The study suggests adopting deep learning models for predicting atmospheric PM2.5 in Bangladesh.
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