Preeclampsia is a condition that might severely impact the health of mothers and their newborns. The aim of this investigation is to examine hematological parameters in mothers with preeclampsia and umbilical cord blood. Eighty preecalmptic mothers were recruited in the study. In addition, eighty normal pregnant mothers served as controls. Hematological parameters that include hemoglobin (Hb), red blood cell count (RBC), red cell distribution width (RDW), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell counts (WBC), platelet counts, mean platelet volume (MPV) and Platelet large cell ratio (PLCR) were examined. Results showed a strong association between preeclampsia and low birth weight, premature/cesarean delivery and proteinuria (P < 0.001). Hb and neutrophils were significantly lower (P < 0.01), whereas RDW, PCV, MCV, MCH, MCHC and lymphocytes were significantly higher than normal ones (P < 0.01). When cord blood of preeclamptic mothers were compared with that of normal ones, similar findings were observed. In addition, results showed significant and positive correlations between preeclamptic mothers and their newborn in Hb (r2 = 0.075, P < 0.05), PCV (r2 = 0.084, P < 0.01), MCV (r2 = 0.077, P < 0.05), MCHC (r2 = 0.115, P < 0.01), RBC (r2 = 0.086, P < 0.01) and retics (r2 = 0.306, P < 0.01). In conclusion, changes in several hematological parameters associated with preeclampsia were correlated in affected mothers and their newborns. Such biomarkers can be used to predict pregnancy outcomes in women with preeclampsia.

Background: There are subtle and substantial changes in hematological parameters during pregnancy and the puerperium total blood volume increases by about 1.5 liter mainly to supply the needs of the new vascular bed (Taylor, 1979). Pregnant places extreme stresses on the hematological system and understanding of the physiological changes that result as obligatory in order to interpret any need for therapeutic intervention. (William and Cindy 2005). Iron deficiency is the most common cause of anemia in pregnancy (Odekule 2010). As consequences various quantitative and qualitative hematological changes occur during pregnancy including cell counts, hemoglobin levels, hematocit, leucocytes, thrombocytes, red blood cells indices, morphological changes and reticulocyte production index. (Lewis et al. 2001). Methods: This a case control study in which 100 pregnant women were enrolled as study group and 50 non pregnant healthy women as control subjects. The study was conducted at Omdurman Al Saudi Maternity Hospital in period from 5/3/ 2011 to 6/6/ 2011 to evaluate hematological parameters of Sudanese pregnant and to establish classification of anemia's on bases of complete blood count (CBC), reticulocyte production index (RPI) and peripheral blood picture. Results: The study revealed that there were significant decreased in RBCs count, hemoglobin (Hb) and packed cell volume (PCV) of pregnant women compared to non pregnant women (P value <0.05) and significant decreased in mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) of pregnant women (P value <0.05). TWBCs count was increased significantly (P. value < 0.050) in contrast platelets count significantly lower than the normal control (P. value <0.05). On bases of blood picture we classified anemia's of pregnancy as normocytic normochromic 37 (37%) with RPI mean value of 0.49±0.2, microcytic hypochromic 52(52%) with RPI mean value of 0.76±0.6, and dimorphic picture11 (11%) with RPI of mean value 2.1±0.8. Conclusion:We observed significant decreased in RBC count, hemoglobin, PCV, red cell indices and platelet count during pregnancy and significant increased in leukocyte count of pregnant women when compared to non pregnant women. We found the most common type of anemia among Sudanese pregnant women is microcytic hypochromic type and likely to be of iron deficiency second class is normocytic normochromic type, and less of occurrence type is dimorphic picture types with increased reticulocyte production index results from prophylaxes iron response.

AimIn this study, we aimed to analyze the genetic and acquired risk factors for deep vein thrombosis (DVT) of the lower extremities among Sudanese women.MethodsA total of 136 women were enrolled in the study, including 75 DVT patients and 61 healthy controls. Demographic and clinical data were collected using a specific questionnaire. Citrated blood samples of patients and controls were used for coagulation assays, and DNAs isolated from EDTA-blood samples were used for the detection of Factor V Leiden and prothrombin G20210A mutations using multiplex polymerase chain reaction-restriction fragment length polymorphism analysis.ResultsBoth gene mutations were found to be absent from all 136 subjects, and therefore did not account for the incidence of DVT in Sudanese women. Of the 75 DVTs, 70 (93.3%) were localized in the left leg and 5 (6.7%) in the right leg. Additionally, 84% of the DVTs were proximal and 16% were distal. Among the 75 patients, 22 (29.33%) were postpartum, 7 (9.33%) were pregnant, and 46 (61.33%) were nonpregnant. Levels of prothrombin fragment 1+2, prothrombin time, activated partial thromboplastin time, and D-dimer were significantly higher in DVT patients than in healthy controls (P<0.0001).ConclusionRisk factors that most significantly affected patients in the 18–45 years age group were pregnancy and oral contraceptive usage, whereas those that most significantly affected patients in the 66–90 years age group were immobility, heart disease, and history of DVT.

Thrombophilia may be anticipated by single or combined hereditary defects in encoding genes factor V, Prothrombin, and MTHFR. The aim of this study was to determine the prevalence and associated risks of V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T) mutations in Saudi women with Deep Vein Thrombosis (DVT) and women with recurrent pregnancy loss (RPL). Protein C and protein S activity were measured to determine combined effects, if any. We examined 60 women with a history of DVT and 60 with RPL, extracted DNA from EDTA blood and determined three mutations by using multiplex PCR reactions followed by Strip Assay KIT. Pro C Global assay was used to determine the cutoff value [PCAT-NR = 0.80]. Protein C/S chromogenic assay was used to estimate protein C and S percentages. Frequency of Factor V Leiden G/A genotype in patients with DVT 7 (11.6%) had a significant association for DVT χ2 (OR = 5.1, P = 0.03). In women with RPL the three mutations did not show any significant association, levels of Protein C, protein S and PCAT-NR in patient groups not different from controls (P > 0.05). In conclusion, we recommend expanding on these data to provide larger-scale studies.