Hematopoietic cell transplantation (HCT) conditioned using myeloablative conditioning (MAC) is complicated by end organ injury due to endothelial dysfunction and graft versus host disease. Mucositis and oxidant injury results in micronutrient deficiency. Ascorbic acid (AA) levels were measured in 15 patients undergoing HCT conditioned with MAC (11 allogeneic and 4 autologous HCT). Ascorbate levels declined post conditioning to 27.3 (±14.1) by day 0 (p <0.05 compared with baseline), reaching a nadir level of 21.5 (±13.8) on day 14 (p <0.05) posttransplant. Patients undergoing allogeneic HCT continued to have low AA levels to day 60 post transplant, whereas recipients of autologous HCT recovered plasma AA levels to normal. The role of AA in maintaining endothelial function and hematopoietic as well as T cell recovery is provided, developing the rationale for repletion of vitamin C following HCT.
In this study, we used a rapid, highly-sensitive, single-cell biomass measurement method, Live Cell Interferometry (LCI), to measure biomass in populations of CD3 + T cells isolated from hematopoietic stem cell transplant (SCT) patients at various times pre- and post-transplant (days 0-100). CD3 + T cell 'mass spectra' were obtained from five autologous and 20 allogenic transplant recipients. We found a pronounced rise in median T cell biomass (+25%; p <0.001) shortly after transplant (day 14), which moderated by day 60. Further, the inter-patient and intra-patient cell masses were most variable at days 14 and 30 post-transplant. T cell biomass trends were similar in both autologous and allogenic transplant recipients. These data suggest that T cell biomass changes are associated with immune reconstitution occurring in the first few weeks post-transplant. To our knowledge, this is the first time single-cell biomass measurements have been studied in human clinical trials. With refinement, these data may prove useful in guiding the withdrawal of immunosuppression following SCT, reducing the likelihood of Graft-Versus-Host Disease or cancer relapse occurring.
receiving LM and support preliminary data that lenalidomide may augment vaccine responses. Conclusions: Re-immunization with inactivated vaccines in patients on LM is both safe and effective, offering this population immunity to vaccine preventable diseases.
Hematopoietic cell transplantation (HCT) conditioned using myeloablative conditioning (MAC) is complicated by end organ injury due to endothelial dysfunction and graft versus host disease. Mucositis and oxidant injury results in micronutrient deficiency. Ascorbic acid (AA) levels were measured in 15 patients undergoing HCT conditioned with MAC (11 allogeneic and four autologous HCT). Ascorbate levels declined postconditioning to 27.3 μMol/L (±14.1) by day 0 (P = .03 compared with pretransplant baseline), reaching a nadir level of 21.5 (±13.8) on day 14 (P = .003) post‐transplant. Patients undergoing allogeneic HCT continued to have low AA levels to day 60 post‐transplant. The role of AA in maintaining endothelial function and hematopoietic as well as T‐cell recovery is provided, developing the rationale for repletion of vitamin C following HCT.
Background: Doxorubicin (Dox) nowadays is a widely used chemotherapy, however using it has a lot of side effects on different body organs. Unfortunately Nephrotoxcisty is one of its common side effects. Aim of Work: In this article we tried to seek for the effect of Vitamin E (Vit E) as a commonly used vitamin to reduce Dox induced nephrotoxisty. Material and Methods: 40 albino rats were involved, they were divided into 4 groups: Control group, Dox group in which Dox was given in a dose of 2.5mg/kg, 3 times/week, Vit E group in this group rats were given Vitamin E alone for 250mg/kg/day daily via intramuscular injection, Dox + Vit E rats in this group received both medications in the previously mentioned doses. At the end of the experimental period blood samples were collected for biochemical analysis of serum Urea, Creatinin and 8-Hydroxy-2´-deoxyguanosine (OHdG). Renal tissues were collected for analysis of tissue OHdG, histological examination (H & E) and immunohistochemical analysis of renal tissue caspase 3 levels. Results: Our results showed deterioration of all parameters and increased immunohistochemical expression of caspase 3 in Dox treated group. On the other hand administration of Dox with Vit E significantly improved all measured parameters and lowers the expression caspase 3 levels. Conclusion: Combined treatment of Dox with Vitamin E decreases risk of Dox induced nephrotoxcisty. Protective effects of Vitamin E can be due its antioxidant and its antiapoptotic actions.
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