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The purpose of this study is to determine limit loads for pipe elbows subjected to in-plane bending moments that tend to close the elbow (i.e., decrease its radius of curvature), and the influence of internal pressure on the value of the limit load. Load-deflection curves were obtained, and from these curves plastic collapse or instability loads at various values of internal pressure were determined. This was done for different pipe bend factors (h = Rt/r2) using the nonlinear finite element analysis code (ABAQUS) with its special elbow element. The limit load was found to increase and then decrease with increasing pressure for all the elbow geometries studied.

The modeling and simulation of the welding process has been of main concern for different fields of applications. Most of the modeling of such a problem has been mainly in 2-D forms that may also include many sorts of approximation and assumptions. This is due to limitations in the computational facilities as the analysis of 3-D problems consumes a lot of time. With the evolution of new finite element tools and fast computer systems, the analysis of such problems is becoming in hand. In this research, a simulation of the welding process with and without metal deposition is developed. A new technique for metal deposition using element movement is introduced. It helps in performing full 3-D analysis in a shorter time than other previously developed techniques such as the element birth.

Background:AmpC type β-lactamases are commonly isolated from extended-spectrum Cephalosporin-resistant Gram-negative bacteria. Also, resistance appeared in bacterial species not naturally producing AmpC enzymes. Therefore, a standard test for the detection of the plasmid-mediated AmpC enzyme and new breakpoints for extended spectrum Cephalosporins are urgently necessary.Objectives:To detect plasmid and chromosomal mediated AmpC-β-lactamases in Gram negative bacteria in community and hospital acquired infections.Materials and Methods:1073 Gram negative clinical isolates were identified by the conventional methods and were screened for AmpC production using Cefoxitin discs. Confirmatory phenotypic identifications were done for the Cefoxitin-resistant isolates using Boronic Acid for combined and double disc synergy tests, Cloxacillin based double disc synergy test, and induction tests. The genotypic identification of plasmid-mediated AmpC was done using multiplex PCR. ESBL production was also screened by discs of Ceftazidime and Cefotaxime with and without Clavulanic Acid (10 μg).Results:The AmpC-producing isolates among all identified Gram negative bacilli were 5.8% (62/1073) as detected by screening disc diffusion methods, where 72% were positive for AmpC by combined disc method (Cefotetan and Boronic Acid), 56.5% were positive by each of Boronic Acid and Cloxacillin double disc synergy tests, 35.5% were positive by the induction test, and 25.8% were plasmid-mediated AmpC β-lactamase producers by the multiplex PCR. Plasmid-mediated AmpC genes retrieved, belonged to the families (MOX, FOX, EBC and CIT). ESBL producers were found in 26 (41.9%) isolates, 15 (57%) of which also produced AmpC. Isolates caused hospital acquired infections were (53/62); of which (39/62) were AmpC producers. While only (8/62) of the isolates caused community-acquired infections, were AmpC producers, and (1.6%) (1/62) were non AmpC producer.Conclusions:The AmpC β-lactamases detection tests had to be included in the routine microbiology workup of Gram negative bacteria, namely Cefoxitin as a screening test, combined Boronic Acid disc test with Cefotetan, followed by synergy tests and finally by the induction test for phenotypic identifications. Multiplex PCR can successfully detect the plasmid AmpC genes.

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