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Many changes have been made in the healthcare practice environment. Understanding of quality practice environment in hemodialysis units has certain implications for maximizing outcomes for clients, nurses, and systems. Developing quality practice environments takes time and commitment to promote and support patients’ safety. Hence improving safety patient culture is vital in dialysis units because it requires for reducing risks for harm, errors of patients and delivering high quality of patients care. The Study aimed to determine the perception of nursing staff’ toward quality practice environment and patients’ safety at Hemodialysis units. Methodology, data collection was utilized a descriptive correlational design for this study, all nursing staff amounted to (n= 90) They are classified into: all head nurses n = 7,, and all nurses who have either diploma (n = 40) or baccalaureate degree (n = 43) who are affiliated to all hemodialysis units (n =7) at Ministry of Health ,Egypt. A package composed of two instruments was used, namely: Environment Scale-Nursing Work Index (PES-NWI) and Hospital Survey on Patient Safety Culture (HSPSC). Results, the major findings indicated that there is a positive correlation significant among practice work environment and patient safety culture except for staffing and resource adequacy in all hemodialysis units of Ministry of Health Hospitals. Also, results point out that the organizational structure of the Ministry of Health Hospitals is characterized by unhealthy environment and unsafe climate that force the nursing staff to have low perception toward most of quality practice environment and patient safety culture factors. The study recommended that initiating a blame-free reporting system to prevent re-occurrence of problems and actions to eliminate them from the workplace by detecting, evaluating, preventing and treating safety work environment