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Tribhuvankirti Rasa is a herbo-mineral Ayurvedic medicine regularly used to treat different types of fever. It has antipyretic and analgesic activities. It is an effective medicine for the common cold, flu and other Vata kapha problems. Laghumalini Vasanta is also Ayurvedic medicine, used to treat chronic fever and effective in pitta disorders. Ministry of AYUSH, Government of India also recommended these medicines to prevent the severe conditions of Cov-2 infection. Review of literature suggested that phytochemical and elemental characterization parameters of Tribhuvankirti Rasa and Laghumalini Vasant are not reported. The objective of this study is to report phytochemical and elemental profiling and to standardize Tribhuvankirti Rasa (TKR) and Laghumalini Vasant (LMV) to confirm quality and purity. Tribhuvankirti Rasa and Laghumalini Vasant evaluated for phytochemical and elemental parameters by HPTLC and ICP-OES respectively. HPTLC analysis confirms LMV contains Piperine and TKR contains Piperine and 6-Gingerol. The solvent systems toluene: ethyl acetate (7: 3) v/v for Piperine & Hexane: Ethyl acetate: Formic acid (4 : 6 : 0.1) v/v for 6-Gingerol were optimized. ICP-OES analysis confirms presence of Zn in LMV and Hg in TKR. HPTLC and ICP-OES methods were validated successfully for Tribhuvankirti Rasa and Laghumalini Vasant. The characterization and method validation parameters presented in this paper may serve as standard reference for quality control analysis of Tribhuvankirti Rasa and Laghumalini Vasant.