The synthesized gold nanoparticles using indole-3-carbinol (AuNPI3Cs) has been characterized and its antineoplastic activities has been studied here. Several techniques have been used to characterize the AuNPI3Cs. Ultraviolet spectroscopy studies indicated the stability of the synthesized AuNPI3Cs, while FTIR analysis proved that indole-3-carbinol was playing an important role in stabilizing the AuNPI3Cs.TEM analysis study showed that AuNPI3Cs were mostly spherical in shape with an average particle size of 3 nm. The selected area electron diffraction pattern exhibited the crystalline nature of AuNPI3Cs, which was further proved by XRD studies. The present study describes the in vitro antineoplastic efficacy of AuNPI3Cs against Ehrlich ascites carcinoma (EAC) cells. Results showed that the IC 50 dose of AuNPI3Cs was significantly capable of elevating intracellular reactive oxygen species. AuNPI3Cs induced apoptosis by increasing the G 2 /M population, LIVE/DEAD cytotoxicity, chromatin condensation, DNA laddering and TUNEL-positive cells significantly (p < 0.001). Reduction of the mitochondrial potential by AuNPI3Cs was substantiated by JC-1 fluorescent staining. These findings will enlighten future biomedical applications of gold nanoparticles using indole-3-carbinol (AuNPI3Cs) as an antineoplastic agent. Drug preparation from AuNPI3CAuNPI3Cs (1 mg ml À1 stock) were prepared by concentrating the solution with RPMI media. The solution of AuNPI3Cs was then sonicated and was used for the assessment of biopharmacological activities.56436 | RSC Adv., 2016, 6, 56435-56449This journal is

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Objective:The aim of the present study was to investigate the socioeconomic, anthropometric status and health morbidity profile of tribal and non tribal female brick-field workers of Paschim Medinipur district. Study design: Assessment of socioeconomic, health and nutritional parameters were done using 300 tribal & non tribal brick-field female workers at the age group of 18-30 years after randomized sampling. Place and duration of study: The study was done in 30 brick-fields located in Paschim Medinipur district of West Bengal in the month of November and December, 2013 for a period of 60 days. Methodology: Through oral questionnaire methods, socioeconomic studies, health morbidity profile and dietary assessments were performed. Anthropometric parameters were measured by the conventional anthropometric methods. Results: From the study it was observed that most of the women workers were illiterate. Female workers of non tribal and tribal groups engaged in this industry were living below the poverty line and the tribal workers showed poor economic status than the non-tribal women workers. The female workers showed significant decrease in height, weight and body mass index, compared to the Indian national standard values. Mid upper arm circumference (MUAC) and waist-hip ratio (WHR) was also significantly lower to the Indian national standard in both age groups of female workers. The intake of energy, protein, carbohydrate carotene, riboflavin, dietary fiber, calcium and iron were significantly lower compared to the standard ICMR RDA. Conclusion: The female workers engaged in brick manufacturing works are mostly illiterate, economically backward, which make them vulnerable to health insecurity. Malnutrition among tribal & non tribal female workers is a problem that has perhaps been under-recognized, and should now take greater priority.