In this paper, we present the results of the use of bifunctional polymeric films of polystyrene (10.3 KD-49.5 KD) to anchor oligo sequences of various lengths (15, 35 and 70-mer). The polymers were prepared by radical polymerization with 4,4'-azobis(4-cyanovaleric acid) as initiator and 3-Carboxy PROXYL to control the molecular weight and polydispersity. They were further modified with N-hydroxysuccinimide to anchor the (5'-AmMC 12 ) oligos. The anchoring reaction was done on a polymer-modified glassy carbon electrode. The probes were hybridized with their ferrocene-labeled complementary sequences. The hybridization reaction was followed by Osteryoung square wave voltammetry (OSWV). The calibration curve showed a narrow and sharp linear range between (5.7-8.0) 10 À7 M and a detection limit around 0.55 mM.
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