We present a rapid
method based on 2D thin layer chromatography
(TLC) for the simultaneous separation and quantification of organic,
lipophilic extractives occurring in lignocellulosic biorefineries.
The separation is based on alkaline followed by acidic elution for
a 2D separation of the different compound classes (sterols, fatty
alcohols, fatty acids, and resin acids). Staining with Nile Blue A
and subsequent scanning and image analysis of the plates provides
quantitative information on the concentration of each compound class.
Correlation coefficients between 0.92 (abietic acid, resin acid) and
0.98 (β-sitosterol, sterol) are determined in these experiments.
Besides the analysis of the composition, this procedure indicates
the relative polarity as the acidity or basicity of the constituents.
TLC results of an industrial lipophilic extractives sample correlate
with state-of-the-art GC × GC–MS measurements with differences
being in the range between 0.5 (fatty alcohols) and 6% (resin acids),
respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.