The microbiological diversity of cultivable bacteria was analyzed in an aerated facultative lagoon. The removal of specific compounds and measures of pollutant load was evaluated with isolated native bacteria, selected and identified in kraft cellulose effluent. The system was operated with an organic loading rate of 0.2 kgCODm-3d-1 for 60 days. Analyses of the fluorescence excitation-emission matrix, acute ecotoxicity, and microbiology were performed. Bioaugmentation tests were done to emphasize the removal of color, using promising species. The removals of biochemical oxygen demand, chemical oxygen demand, and total organic carbon in AFL were 94%, 51%, and 41%, respectively. Regarding color, removal was up to 4%, and the total phenolic compounds were not removed through biological treatment. The treatment also decreased turbidity by 94% and lignin derivatives by 12%. The bacteria identified through NCBI-BLAST and statistical similarity totaled 9 species in the cellulose effluent, three of which have the potential for color treatment: Bacillus cereus, Bacillus thuringiensis, and Paenibacillus sp. The Bacillus cereus combined with biomass removed color (69%), total phenolic compounds (37%), and compounds derived from lignin (53%). These species are promising for removing specific parameters combined with biomass from biological AFL treatment systems.
The microbiological diversity was evaluated in an aerated facultative lagoon system with organic loading rates of 0.2 and 0.6 kgCODm-3d-1 treating effluent from the kraft pulp industry for bioaugmentation purposes. Samples were taken from a laboratory-scale lagoon at steady state treating kraft pulp effluent and operated with two different rates for 120 days. The analysis was performed by 16S rDNA sequencing. The autochthonous bacteria were identified through statistical similarity obtained from the NCBI-BLAST database. The lagoon performance was assessed based on the removal efficiency of biochemical oxygen demand (94% and 80%), chemical oxygen demand (51% and 44%), total organic carbon (49% and 41%), lignin-derived compounds (13% and 27%), turbidity (94% and 87%), for the low and high rates used respectively. Color and TPC were not significantly removed during the biological treatment of the industrial matrix. In the biomass samples collected at a steady state, 9 and 12 species of bacteria were identified at the applied rates. The species Bacillus cereus, Bacillus thuringiensis, and Paenibacillus glucanolyticus found in this matrix presented significant removal of the parameters in the kraft effluent. The three referred species show great promise in the removal of specific parameters in an AFL biological treatment system using bioaugmentation.
Background: This study analyzes the microbiological diversity in an aerated facultative lagoon system with volumetric organic loading rates of 0.2 and 0.6 kgCODm-3d-1 treating effluent from the kraft pulp industry through bioaugmentation. The samples for the study of biological diversity were taken from a laboratory-scale lagoon at steady state treating kraft pulp effluent and operated with 0.2 and 0.6 kgCODm-3d-1 for 120 days. This analysis was performed by identifying the 16s DNA sequencing, through DNA extraction, polymerase chain reaction and agarose gel electrophoresis at 1%. Next, the autochthonous bacteria were named through statistical similarity obtained from the National Center for Biotechnology Information database. The lagoon performance was assessed based on the removal efficiency of specifics compounds.Results: In the biomass samples collected at steady state, 9 and 12 species of bacteria were identified and the species Bacillus cereus, Bacillus thuringiensis and Paenibacillus glucanolyticus found in this matrix presented significant removal of the parameters in the kraft effluent. In the treatment, it was possible to observe that the removal of organic matter above 50% for chemical demand and above 95% for biochemical demand. The specific compounds were not significantly removed, but this is a characteristic of biological treatments. Conclusions: We found that the three referred species show great promise in the removal of specific parameters in a lagoon biological treatment system using bioaugmentation.
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