Results indicated that ASIs of PVF and VI determined via analysis of pressure plate measurements were reliable indicators of clinical lameness in dogs, but the ASI of PVP was not. The ASI of PCA is an interesting new variable for assessment of limb loading symmetry.

a b s t r a c tSubtle lameness in horses may be difficult to diagnose and methods to evaluate lameness objectively are useful when equine clinicians fail to reach a consensus. The aim of this study was to determine whether equine pressure plate measurements are repeatable when used to calculate forelimb loading (peak vertical pressure [PVP], peak vertical force [PVF], vertical impulse [VI]) and symmetry ratios, and to establish if these data are similar to the 'gold standard' force plate values. Since plate dimensions are relatively small, ponies were used to enable recordings to be taken from both forelimbs in one trial. Five sound ponies were walked and trotted over a pressure plate which was embedded in a custom-made runway. For each pony, five valid trials were recorded during two different days. A trial was considered valid if complete prints of both fore hooves were recorded consecutively and if velocity was within a preset range.The PVP, PVF and VI values showed an acceptable variability (CV 6 16%), with PVF (130% of bodyweight [BW], n = 5) similar to previously reported force plate data (128% BW, n = 48). Mean symmetry ratios appeared to be high (>95%) and showed a low variability (CV < 5%). The stand-alone pressure plate permitted adequate registration of forelimb PVF, VI and limb symmetry with a high level of precision in sound ponies.

Summary Background There is a need to improve therapies for osteoarthritis in horses. Objectives To assess the efficacy of equine allogeneic chondrogenic‐induced mesenchymal stem cells combined with equine allogeneic plasma as a novel therapy for osteoarthritis in horses. Study design Randomised, double‐blinded, placebo‐controlled experiment. Methods In 12 healthy horses, osteoarthritis was induced in the metacarpophalangeal joint using an osteochondral fragment‐groove model. Five weeks after surgery, horses were randomly assigned to either an intra‐articular injection with chondrogenic‐induced mesenchymal stem cells + equine allogeneic plasma (= intervention) or with 0.9% saline solution (= control). From surgery until the study end, horses underwent a weekly joint and lameness assessment. Synovial fluid was collected for cytology and biomarker analysis before surgery and at Weeks 5, 5 + 1d, 7, 9 and 11. At Week 11, horses were subjected to euthanasia, and the metacarpophalangeal joints were evaluated macroscopically and histologically. Results No serious adverse events or suspected adverse drug reactions occurred during the study. A significant improvement in visual and objective lameness was seen with the intervention compared with the control. Synovial fluid displayed a significantly higher viscosity and a significantly lower glycosaminoglycan concentration in the intervention group. Other biomarkers or cytology parameters were not significantly different between the treatment groups. Significantly less wear lines and synovial hyperaemia were present in the intervention group. The amount of cartilage oligomeric matrix protein, collagen type II and glycosaminoglycans were significantly higher in the articular cartilage of the intervention group. Main limitations This study assessed the short‐term effect of the intervention on a limited number of horses, using an osteoarthritis model. This study also included multiple statistical tests, increasing the risk of type 1 error. Conclusions Equine allogeneic chondrogenic‐induced mesenchymal stem cells combined with equine allogeneic plasma may be a promising treatment for osteoarthritis in horses. The Summary is available in Spanish – see Supporting Information

a b s t r a c tModern pressure plates (PP) could be an alternative to traditional force plates (FP) for quantitative equine gait analysis, thereby providing the clinician with objective data on the horse's gait while unravelling the loading of different regions of the hoof during the stance phase. The aim of this study was to determine whether a stand-alone PP allows reliable measurement of gait kinetics, compared to simultaneously recorded FP variables. Six sound Warmblood horses were walked and trotted over a combined PP and FP system for collection of a set of five valid kinetic measurements for each forelimb. A measurement was considered valid if the horse was moving in a straight line at a constant pace while gait velocity was within a preset range and the hoof fully contacted the plate surface.Significant differences between FP and PP data were seen for peak vertical force (PVF), vertical impulse (VI), time at which the PVF occurs (tPVF) and forelimb symmetry ratios (SymPVF and SymVI) (P < 0.05), but not for stance phase duration (ST). Nevertheless, mean agreement indices (AIs) of ST, tPVF and SymP-VF and SymVI were excellent (P0.92), whereas AIs of PVF and VI were moderate (P0.70). The excellent agreement between PP and FP symmetry ratios confirms that observed differences between PP and FP in symmetry ratios are small (2-7%), especially when compared to the expected decrease in symmetry associated with mild lameness (>20%). The results indicate that a stand-alone pressure plate can be used to measure absolute (ST) and relative (tPVF) temporal variables and loading symmetry ratios and offers equine veterinarians a mobile, cost-efficient and quick gait evaluation method for routine clinical use. However, the system cannot be used interchangeably with a force plate to measure absolute values of limb loading.

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Visual equine lameness assessment is often unreliable, yet the full understanding of this issue is missing. Here, we investigate visual lameness assessment using near-realistic, three-dimensional horse animations presenting with 0–60 per cent movement asymmetry. Animations were scored at an equine veterinary seminar by attendees with various expertise levels. Results showed that years of experience and exposure to a low, medium or high case load had no significant effect on correct assessment of lame (P>0.149) or sound horses (P≥0.412), with the exception of a significant effect of case load exposure on forelimb lameness assessment at 60 per cent asymmetry (P=0.014). The correct classification of sound horses as sound was significantly (P<0.001) higher for forelimb (average 72 per cent correct) than for hindlimb lameness assessment (average 28 per cent correct): participants often saw hindlimb lameness where there was none. For subtle lameness, errors often resulted from not noticing forelimb lameness and from classifying the incorrect limb as lame for hindlimb lameness. Diagnostic accuracy was at or below chance level for some metrics. Rater confidence was not associated with performance. Visual gait assessment may overall be unlikely to reliably differentiate between sound and mildly lame horses irrespective of an assessor’s background.

The efficient and fast isolation of microorganisms from infected SF by the BACTEC system allows for rapid susceptibility testing and a more appropriate antibiotic treatment.

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