Thermostable alkaline cellulases can resist high temperature and high pH, which make them widely applicable in the industries of food, detergent, paper making, and biomass utilization. To obtain favorite thermostable alkaline cellulaseproducing bacterial strains, these bacteria were isolated from collected soil and plant putrefaction samples in long-term garbage dumps. One bacterium strain from lime-applied garbage dumps, named as SWU-27, was picked up since its crude enzyme showed stronger activities of cellubiohydrolase, endoglucanase, and β-glucosidase. All these activities could tolerate the treatment of high temperature (up to 80°C) and endoglucanase and β-glucosidase activities could even tolerate high pH (over 10). Taxonomic study showed that strain SWU-27 is another geographic strain of Gram-negative Klebsiella pneumoniae. In conclusion it is suggested that lime-applied dumps are preferable places to isolate novel thermostable alkaline cellulaseproducing bacterial strains.
Chitinases are employed to the conversion of chitin and are produced by a wide range of bacteria. The objective of this study was to isolate chitinase-producing microorganisms with high chitinolytic activity. A thermostable alkaline chitinase producing isolate strain CQNU6-2 was obtained from soil samples and showed potential in biodegradation of shrimp shell wastes. The optimal culturing conditions of isolate CQNU6-2 is at 25°C and pH 7 for 24 h. The chitinase produced by strain CQNU6-2 exhibited maximum activity at pH 6.0 and 40°C and it could tolerate the treatment of high temperature (up to 80°C) and high pH (over 10). Taxonomic study, based on biochemical and morphological analysis and phylogenetic analysis of 16S rDNA, showed that strain CQNU6-2 was belongs to the genus Aeromonas sp. The isolate can effectively hydrolyze colloidal chitin with degradation rate of 100% and also can directly degrade the shrimp shells. Ammonium sulfate precipitation method can be used to preliminary purify the chitinase. In conclusion, strain CQNU6-2 had a promising potential for biodegradation of chitin under harsh pH or temperature conditions and could be employed to the comprehensive utilization of shrimp shell wastes.
The traditional Pu'er tea fermentation is uses the man-power to moisten naturally in the workshop piles the fermentation, this fermentation way cannot guarantee that in the Pu'er tea fermentative process the humidity uniformity, produces the workshop and the Fermentation plant to the Pu'er tea carries on the design and the research, the microorganism technology, the machine-finishing technology and the electric control technology merges into one organic whole, causes the Pu'er tea the production standardization, and has the scientific nature. The Pu'er tea fermented the clear purification workshop to unify the technology which the tradition fermented, used modernized the examination control technology and the equipment, by humidity sensor, PLC master control equipment, computer constitutions and so on processing system, Canadian wet equipment and supervisory system. This article through constructs the Pu'er tea to ferment the clear purification workshop, carries on the controlto the workshop ambient humidity, causes the Pu'er tea to be stable in fermentative process environment, and has carried on the production testing under this kind of environment, obtains the experimental correlation data and carries on processing, discovers the Pu'er tea fermentation humidity from the tentative data to form the influence to the Pu'er tea quality some rules, ferments the formalization production for the Pu'er tea to provide the corresponding scientific parameter.
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