Toxicity of neem extract (RB-a) against Anopheles stephensi (Mosquito) and Aphanius dispar (Killifish) was studied. The applied concentration of RB-a against Aphanius dispar were 20,30,40,50 and 60 ppm, while the mortality percent was 6.67, 36,67,53.33,80 and 90% respectively. The LC 50 was found to be 36.37 ppm and LC 90 was found to be 61.04 ppm. The applied concentrations of RB-a were 30,60,90,120 and 150 ppm against Anopheles stephensi, the mortality percent were observed as 15,40,60.80 and 85% respectively. The LC 50 against this pest was found to be 69.4 ppm and LC 90 , was found to be 182.77 ppm.

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