Background: Inadequate temperature affects the stability of intact parathyroid hormone (i-PTH) kits. Room temperature during transport modifies i-PTH results. Method: Percent bound (%B/Bmax) and concentrations ( pmol/L) of standards, controls (C1, C2) and pool from eight standard curves were divided into: group I (three curves from kits kept at room temperature for more than 48 h) and group II (five curves from kits kept at 2 -88C) during transport. i-PTH was measured using Scantibodies total i-PTH assay with RIAMAT-280. Results: %B/Bmax for standards, C1 and C2 were significantly higher in group I versus II (P ¼ 0.04). %B/Bmax for the pool were significantly lower in group I (P ¼ 0.001). i-PTH pool concentration in group I was 51% lower (95% confidence interval, 47-53%, P ¼ 0.001); differences were not significant for C1 (P ¼ 0.25) and C2 (P ¼ 0.57) in both groups. Conclusion: Room temperature on i-PTH kit during transport alters the standard curve, resulting in a decrease in i-PTH. Using a pool as internal quality control allows the detection of these changes not detected by kit controls.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.