The routine availability of nucleated human cells for experimental use in limited in the absence of venipuncture. In this paper we have demonstrated that macrophages may be harvested routinely from the waste dialysis bags of patients undergoing continuous ambulatory peritoneal dialysis. These cells were identified as macrophages by morphology, adherence, phagocytosis, chemotaxis, non-specific esterase staining and peroxidase staining. Macrophages from patients with end-stage renal disease produced arachidonate cyclo-oxygenase products in a pattern similar to that of ascites macrophages obtained from patients with normal kidney function. Arachidonate metabolism was shown to be manipulatable. Thus, indomethacin blocked synthesis of cyclooxygenase products, and OKY-1581, a specific thromboxane synthase inhibitor, increased the release of prostaglandin E2 and prostacyclin, measured as its stable breakdown product 6-keto-prostaglandin F1, whereas the thromboxane B2 synthesis was effectively inhibited.
have bred and studied a strain of Wistar rats which spontaneously develops hypertension ( 1 , 2). Early in life, the blood pressure of the spontaneously hypertensive rat is no different from that of control (C) Wistar rats, but with the passage of time, the blood pressure of SHR's rises steadily. Because of the gradual development of hypertension in the setting of a genetic background, the SHR may be the closest laboratory model of human essential hypertension that we have.Based upon the speculation that some forms of clinical and experimental hypertension are secondary to deranged kidney function (3, 4), one might easily hypothesize that deranged kidney function also may be, at least in part, a primary cause of hypertension in the SHR. In this investigation, we examined the effects of sera and sera fractions from SHR and C rats on para-aminohippurate (PAH) and tetraethylammonium (TEA) transport in kidney cortical slices from control rats. The purpose of our report is to show that plasma obtained from SHR's affect renal transport of PAH and probably TEA in a different manner from that of C rats. The factor or factors demonstrated in this in vitro assay bear in many ways a resemblance to those found in azo-
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