IV PEG-ASP is associated with a significantly higher rate of allergic reactions than IM. The clinical preference for IV PEG-ASP may warrant re-evaluation.
A regimen of gentamicin 6 mg/kg IV every 24 hours for Pediatric Intensive Care Unit/Cardiac Critical Care Unit patients at SickKids weighing ≥5 kg with SCr <20% above age-specific upper normal limit before initiation of gentamicin is proposed.
A set of 140 winter barley genetic resources of foreign and domestic origins was tested on experimental basis of RIPP in 1997RIPP in -1999 to characterise the variability of the accessions based on agronomic data using multivariate methods. In the set tested, variability was studied of selected traits and characteristics such as: plant height (PH), weight of 1000 grains (W), grain number per a spike (SNG), grain uniformity -ratio of front seeds over 2.5 m sieve (GU), vegetation period -sowing/full maturity (VM) and seed yield (Y). Agronomic characters show great variability between cultivars. The study of matrix interrelationships between different variables showed that the yield is greatly correlated with traits: vegetation period -sowing/full maturity, grain uniformity and grain number per a spike. High positive correlation was obtained between the grain uniformity and the weight of 1000 grains. Negative correlation was found between the grain number per a spike and weight of 1000 grains in six-row barley. Correlations between agronomic traits differed between two-and six-row barley sets. The study revealed the existence of genetic differences among accessions as well as differences between two-and six-row winter barley and between the genotypes of domestic and foreign country origin, respectively. Results of this study provided information about diversity which should be of particular interest for the further collecting of genetic resources.Keywords: barley; Hordeum vulgare L.; diversity; morphology; variation; agronomic characters; cluster analyses; principal component; cultivars 119Czech J. Genet. Plant Breed., 40, 2004 (4): 118-126 been used in widely different fields (M����� et al. 1986). This work describes phenotypic diversity observed in winter barley cultivars. A similar work has been carried out with spring barley (Ž����� & Ž�� 1999). The multivariate analysis, and in particular, the principal component and cluster analyses have been utilised for the evaluation of a collection of 140 spring barley genotypes from the Gene Bank of RIPP Piešťany. The main objectives of this study were: (a) to assess the phenotypic diversity for agronomic descriptors of winter barley, (b) to determine the associations between these descriptors, (c) to estimate and compare the phenotypic diversity in two-row and six-row winter barley accessions which are maintained by the genebank in Slovakia, (d) to determine the within-accessions and between-accessions agronomic variations of domestic and non domestic accessions. MATERIAL AND METHODS Plant material.A total of 140 accessions (47 tworow and 93 six-row) of winter barley maintained at the Gene Bank of RIPP Piešťany were studied. They were grown during two years 1997/1998 and 1998/1999 in the experimental fields of the institute. The experiment was set up as a randomised block design.The accessions were evaluated for six agronomic descriptors (Table 1) according to the Descriptors list for barley (A�������� 1994; L���� et al. 1986). The traits not scored in...
The variation in 20 morphological and agronomical traits has been evaluated in a set of chickpea genetic resources from four countries. Data indicated differences between accessions in leaf, flower, pod, and seed traits and characteristics, as well as in vegetation period. Multivariate analyses of these data segregated chickpeas into groups. Polymorphism in seed glutelines was absent, while variation in seed prolamines was very limited. DNA amplification patterns have been analyzed by semi-arbitrary primers and by specific microsatellite primers. Only some of semi-arbitrary primers generated usable DNA banding patterns, moreover interpretation of these patterns can be more or less difficult. On the contrary specific primers amplifying microsatellites at the specific loci generated unambiguous and reproducible differences between chickpeas.
Abstract:The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western and Eastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics. Nevertheless, some of the accessions were identical in these characteristics. Cluster analysis grouped genotypes into two main branches, reflecting the growth type, seed size parameters, and thousand-seed weight. Molecular differentiation studies were performed by multilocus polymorphism detection in microsatellite and minisatellite DNA regions. Cluster analysis based on molecular data also grouped genotypes but no linkage to morphological traits was revealed. Bean accessions with very similar or identical morphological characters were clearly distinguished by DNA banding patterns. The presence of duplicates was excluded.
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