Intraperitoneal administration of tripeptide Gly-His-Lys to male rats in doses of 0.5, 5, and 50 μg/kg 12 min before the start of the experiment produced an anxiolytic effect in the elevated plus maze test manifested in an increase in the time spent in open arms and shortened time spent in the closed arms. The anxiolytic effect was most pronounced after injection of 0.5 μg/kg peptide and decreased with increasing the dose of the peptide. Replacement of L-lysine with D-lysine in the tripeptide molecule was accompanied by a significant weakening of the neurotropic effects in all studied doses. Attachment of D-alanine to N- or C-terminus of Gly-His-Lys peptide leveled its anxiolytic action in all doses; significant changes in some measures of increased anxiety after administration at 50 μg/kg were found.
Ten intraperitoneal injections of tripeptide Gly-His-Lys in doses of 1.5, 5, 50, 150, and 450 mg/kg stimulated mitotic activity of hepatocytes and dose-dependently suppressed immune reactivity (number of antibody-producing cells and delayed-type hypersensitivity reaction).
The aim of the study was to provide a comparative assessment of reparative osteogenesis activity and neutrophil func-tion when thymogen, dalargin, Gly-His-Lys (GHK) peptides and their paired combinations were injected into the experi-mental fracture area. Materials and methods. Peptides were administered to Wistar rats in the area of fracture for ten days from the day of injury. Equimolar single doses of the drugs were used: dalargin - 1.2 µg/kg, while thymogen and GHK were injected in a dose of 0.5 µg/kg weight. Reparative activity of osteogenesis was assessed histologically and radiologically. Phagocytic and oxygen-dependent activity of the blood neutrophils was determined. Results. All peptides and their paired combinations increased the activity of reparative osteogenesis. Even more pro-nounced bone repair activity was observed after the injection of GHK peptide in combination with dalargin or thymogen with maximum intensity in animals treated with combined administration of GHK and dalargin. The fracture of the femur bone in experimental rats was also accompanied by a decrease in phagocytic and oxygen-dependent activity of neutrophils. The peptides increased the indices of neutrophils activity more markedly when using paired combinations of peptides and with the manifestation of a corrective effect in animals receiving combined administrations of GHK and dalargin. Conclusion. Synergistic action of Gly-His-Lys peptides, thymogen and dalargin significantly enhances reparative and stimulating neutrophil function in tubular bone fractures and can be used to activate reparative osteogenesis and neutrophil bactericidal activity.
The mitotic index of hepatocytes remained unchanged after 10 intraperitoneal injections of DSLET and ACTH(4-10) in doses of 0.5, 1.5, and 5 microg/kg, but increased after injection of these substances in doses of 50 and 150 microg/kg. DSLET in doses of 5, 50, and 150 microg/kg decreased the number of antibody-producing cells in the spleen. ACTH(4-10) possessed immunosuppressive activity not only in these doses, but also in a dose of 1.5 microg/kg. As differentiated from mitotic activity of hepatocytes, the degree of immunosuppression increased with increasing the dose of test peptides.
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