Studying the strategies of improving abiotic stress tolerance is quite imperative and research under this field will increase our understanding of response mechanisms to abiotic stress such as heat. The Hsp70 is an essential regulator of protein having the tendency to maintain internal cell stability like proper folding protein and breakdown of unfolded proteins. Hsp70 holds together protein substrates to help in movement, regulation, and prevent aggregation under physical and or chemical pressure. However, this review reports the molecular mechanism of heat shock protein 70 kDa (Hsp70) action and its structural and functional analysis, research progress on the interaction of Hsp70 with other proteins and their interaction mechanisms as well as the involvement of Hsp70 in abiotic stress responses as an adaptive defense mechanism.

Longer fruits, heavy fruits and a high number of fruits are variables that are related to higher yields of chili pepper under tropical conditions and hence could be used as a reliable indicator in indirect selection for yield. © 2016 Society of Chemical Industry.

Backcrossing together with simple sequence repeat marker strategy was adopted to improve popular Malaysian chilli Kulai (Capsicum annuum L.) for heat tolerance. The use of molecular markers in backcross breeding and selection contributes significantly to overcoming the main drawbacks such as increase linkage drag and time consumption, in the ancient manual breeding approach (conventional), and speeds up the genome recovery of the recurrent parent. The strategy was adopted to introgress heat shock protein gene(s) from AVPP0702 (C. annuum L.), which are heat-tolerant, into the genetic profile of Kulai, a popular high-yielding chilli but which is heat sensitive. The parents were grown on seed trays, and parental screening was carried out with 252 simple sequence repeat markers. The selected parents were crossed and backcrossed to generate F hybrids and backcross generations. Sixty-eight markers appeared to be polymorphic and were used to assess the backcross generation; BCF, BCF and BCF. The average recipient allele of the selected four BCF plants was 80.75% which were used to produce the BCF generation. BC-P was the best BCF plant because it had the highest recovery at 83.40% and was positive to Hsp-linked markers (Hsp70-u2 and AGi42). After three successive generations of backcrossing, the average genome recovery of the recurrent parent in the selected plants in BCF was 95.37%. Hsp gene expression analysis was carried out on BCF, BCF and BCF selected lines. The Hsp genes were found to be up-regulated when exposed to heat treatment. The pattern of Hsp expression in the backcross generations was similar to that of the donor parent. This confirms the successful introgression of a stress-responsive gene (Hsp) into a Kulai chilli pepper variety. Furthermore, the yield performance viz. plant height, number of fruits, fruit length and weight and total yield of the improved plant were similar with the recurrent parent except that the plant height was significantly lower than the Kulai (recurrent) parent.

Global demand for food has always been on the increase due to the increase of the population in this world. Intercropping is one of the alternatives of agronomic practices that is widely practiced in ensuring food security and enhancing yield stability. Strip, mixed, and relay intercropping can be practiced to increase crop production. In addition to achieving a successful intercropping system, factors such as suitable crops, time of sowing, maturity of the crop, and plant density need to be considered before and during planting. Besides, practiced intercropping becomes a useful cropping system to increase efficient resource utilization, enhance biodiversity, promote soil health, enhance soil fertility, erosion control, yield advantage, weed, pest, and disease control, insurance against crop failure, ecosystem and modification of microclimate, market instability, and increase farmers income. Crop productivity in any types of cropping system implemented relies primarily on the interception of photosynthetically active radiation (PAR) of crop canopy and conversion of intercepted radiation into biomass or known as radiation use efficiency (RUE). Both PAR and RUE are important measurements that have significant roles in crop growth and development in which the accessibility of these radiation dynamics is connected with the leaf area index and crop canopy characteristics in maximizing yield as well as total productivity of the crop component in intercropping systems.

Background: One of the key importance of vegetable consumption is to obtain sufficient micronutrients, dietary fibre, and for the prevention of childhood obesity. Most Malaysian children did not meet the recommended intake of vegetable consumption, and this is especially vulnerable among the urban poor population due to food insecurity. Efforts are needed to promote vegetable consumption that fall short of the recommended intake level. Aim: This trial aims to examine the effectiveness of the “GrowEat” project, as a nutrition intervention programme integrated with home gardening activities to improve vegetable consumption among urban poor children in Kuala Lumpur, Malaysia. Methods: This is a single-blinded parallel two-arm cluster randomised controlled trial (RCT) that include 134 children. Two zones in Kuala Lumpur will be randomly selected, and three low-cost housing flats from each zone will be selected as the intervention and control groups respectively. The trial is designed based on the social cognitive theory (SCT). Children from the intervention group ( n = 67) will attend a 12-week programme, which consists of home garden-based activities, gardening and nutrition education session. Assessment will be conducted for both groups at three time points: baseline, post-intervention and follow-up phase at 3 months after the intervention. Conclusion: We anticipate positive changes in vegetable consumption and its related factors after the implementation of the “GrowEat” project. The current intervention may also serve as a model and can be extended to other urban poor population for similar interventions in the future to improve vegetable consumption, agriculture and nutrition awareness.

Domestic production of chilli in Malaysia is insufficient which accounted for 52% selfsufficiency level. Poor performance of local varieties under high temperatures above 42°C is one of the major factors affecting chilli production. This study was conducted with the objective of introgress heat-tolerant gene (Hsps) from AVPP0702 donor into Kulai using marker-assisted backcrossing. A total of 68 SSR markers, including Hsp linked markers that showed polymorphism between the parents were used to assess the generation of backcrossing; BC1F1, BC2F1, BC3F1 and BC3F2 and the average RPG percentage of the recurrent parent was found to be 81, 90, 95 and 97%, respectively. The pattern of Hsp expression in the backcross generations was similar to the donor parent (upregulated with more than 4-fold increase). Twelve improved heat tolerant chilli lines were developed. Most of the morphological and agronomical traits were recovered in the selected improved heat-tolerant genotypes from Kulai such as plant height, number of days to 50% flowering, number of fruits, fruit length and total fruit yield per plant. Improved high-yielding heat-tolerant chilli lines showed tolerance to high temperature as well as did not express any negative effect on agronomic traits in comparison with Kulai variety.

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