Aim :Methodology : Results :Interpretation :The aim of the present study was to measure in-vitro DNA and cell damage induced by 5-chloro-2methyl-4-isothiazolin-3-one (CMIT), one of the humidifier disinfectants, and to investigate the suppressive effect of various phytochemicals against CMIT toxicity. In-vitroc o m e t a s s a y w a s performed to determine the degree of CMIT-induced DNA damage at single cell level by measuring the olive tail moment. Upon treating CMIT on the rat lymphocytes, the inhibitory effects of Vitamin C and several phytochemicals s u c h a s b e r b e r i n e , curcumin and resveratrol w e r e a s s e s s e d . I n addition, MTT assay was used to examine the p r o t e c t i v e e f f e c t o f resveratrol on CMITinduced cytotoxicity in cultured lung cells.In in-vitro comet assay, the increased olive tail moment induced by CMIT was effectively inhibited by Vitamin C, berberine, curcumin and resveratrol treatment. Especially, resveratrol showed the best suppressive effect against DNA damage by CMIT. In MTT assay, resveratrol also showed significant suppressive effect against cytotoxicity induced by CMIT in cultured lung cells.Phytochemicals such as Vitamin C, berberine, curcumin and resveratrol can be utilized in the development of preventive or therapeutic compositions against the injury caused by CMIT toxicity.
Triclosan, an antibacterial and antifungal agent, is widely used in several consumer products, including soaps, toothpaste and surgical cleaning treatments. The present study aimed to examine oxidative DNA damage in rat lymphocytes and its protection by phytochemicals via comet assay. DNA damage of rat lymphocytes induced by triclosan was measured by the olive tail moment in the comet assay. Following the addition of N-acetylcysteine, curcumin, berberine and resveratrol, the reduction of DNA damage was observed by using comet assay. The increased olive tail moment induced by triclosan was significantly reduced upon treating N-acetylcysteine and three phytochemicals, such as c u r c u m i n , b e r b e r i n e a n d resveratrol. Notably, the oxidative DNA damage by triclosan was dramatically suppressed by curcumin close to the control value, which means almost complete protection in vitro. These results suggest that in vitro suppressive effect of curcumin, berberine and resveratrol against DNA damage by triclosan might be due to their antioxidative properties, and could be utilized for developing a reducing agent for triclosan toxicity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.