In this study, PFAST was a useful and reliable diagnostic tool when used as part of surgical triage at the trauma scene.

The simulation-based intervention offers a positively evaluated possibility to enhance students' skills in recognising and handling emergencies. Additional studies are required to measure the long-term retention of the acquired skills, as well as the effect of training in healthcare professionals.

SUMMARYThis paper presents the detailed architecture of the WISECOM system, which can quickly re-establish and provide telecommunication services after a disaster by integrating terrestrial mobile radio networks, such as GSM, WiFi, WiMAX and TETRA, with satellite technologies. The system aims to be a useful tool to be deployed in the early hours after a disaster event, for both the victims and the rescue services who will be able to communicate in a reliable and robust way, improving the coordination of the different teams and reducing the time needed to provide victims with the proper treatment. The paper presents in detail the different services provided by the system taking into account its two different versions, based on two different satellite technologies, Inmarsat BGAN and DVB-RCS. Together with the presentation of the system capabilities, a business model is also proposed. Thereafter, the architecture of the general system and the demonstrators that have been developed are detailed, according to the two versions of the system. The work also presents the outcomes of the tests conducted with a prototype of the system, and of the final project demonstration, which was held in Germany in May 2008 with the involvement of real end-users (fire brigades and civil protection authorities).

Confocal laserscan microscopy with a dual-excitation device was used to record intracellular pH (pHi) regulation in rat proximal convoluted tubules microperfused in vivo. Cells were loaded with the pH-sensitive, fluorescent dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Single cells could be distinguished within the tubules and separate measurements were possible. Application of an NH4Cl pulse by peritubular perfusion caused an immediate increase in intracellular pH. Intraluminal injection of NH4Cl led to a slower increase in intracellular pH. In both cases, cessation of perfusion led to an immediate acidification. Peritubular perfusion with 300 microM 4,4'-diisothiocanatodihydrostilbene-2,2'-disulphonic acid (H2DIDS) caused an intracellular alkalinisation. Microperfusion, pH-sensitive dyes and confocal laserscan microscopy provide a new non-invasive method to measure intracellular pH effectively in individual cells of near-surface structures of the intact kidney.